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The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs
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UNSPECIFIED (1997) The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs. FEMS MICROBIOLOGY LETTERS, 156 (2). pp. 205-210. ISSN 0378-1097.
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Abstract
The particulate methane monooxygenase gene pmoA, encoding the 27 kDa polypeptide of the membrane-bound particulate methane monooxygenase, was amplified by PCR from DNA isolated from a blanket peat bog and from enrichment cultures established, from the same environment, using methane as sole carbon and energy source. The resulting 525 bp PCR products were cloned and a representative number of clones were sequenced. Phylogenetic analysis of the derived amino acid sequences of the pmoA clones retrieved directly from environmental DNA samples revealed that they form a distinct cluster within representative PmoA sequences from type II methanotrophs and may originate from a novel group of acidophilic methanotrophs. The study also demonstrated the utility of the pmoA gene as a phylogenetic marker for identifying methanotroph-specific DNA sequences in the environment.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | FEMS MICROBIOLOGY LETTERS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0378-1097 | ||||
Official Date: | 15 November 1997 | ||||
Dates: |
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Volume: | 156 | ||||
Number: | 2 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 205-210 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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