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An immunological approach to detect phosphate stress in populations and single cells of photosynthetic picoplankton
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UNSPECIFIED (1997) An immunological approach to detect phosphate stress in populations and single cells of photosynthetic picoplankton. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 63 (6). pp. 2411-2420. ISSN 0099-2240.
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Abstract
In the marine cyanobacterium Synechococcus sp. strain WH7803, PstS is a 32-kDa cell wall-associated phosphate-binding protein specifically synthesized under conditions of restricted inorganic phosphate (P-i) availability (D. J. Scanlan, N. H. Mann, and N. G. Carr, Mol; Microbiol. 10:181-191, 1993). We have assessed its use as a potential diagnostic marker for the P status of photosynthetic picoplankton. Expression of PstS in Synechococcus sp, strain WH7803 was observed when the P-i concentration fell below 50 nM, demonstrating that the protein is induced at concentrations of P-i typical of oligotrophic conditions. PstS expression could be specifically detected by use of standard Western blotting (immunoblotting) techniques in natural mesocosm samples under conditions in which the N/P ratio was artificially manipulated to force P depletion. In addition, we have developed an immunofluorescence assay that can detect PstS expression in single Synechococcus cells both in laboratory cultures and natural samples, We show that antibodies raised against PstS cross-react with P-depleted Prochlorococcus cells, extending the use of these antibodies to both major groups of prokaryotic photosynthetic picoplankton. Furthermore, DNA sequencing of a Prochlorococcus pstS homolog demonstrated high amino acid sequence identity (77%) with the marine Synechococcus sp. strain WH7803 protein, including those residues in Escherichia coil PstS known to be directly involved in phosphate binding.
Item Type: | Journal Article | ||||
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Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology |
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Journal or Publication Title: | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | ||||
Publisher: | AMER SOC MICROBIOLOGY | ||||
ISSN: | 0099-2240 | ||||
Official Date: | June 1997 | ||||
Dates: |
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Volume: | 63 | ||||
Number: | 6 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 2411-2420 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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