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EVIDENCE THAT PARTICULATE METHANE MONOOXYGENASE AND AMMONIA MONOOXYGENASE MAY BE EVOLUTIONARILY RELATED
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UNSPECIFIED (1995) EVIDENCE THAT PARTICULATE METHANE MONOOXYGENASE AND AMMONIA MONOOXYGENASE MAY BE EVOLUTIONARILY RELATED. FEMS MICROBIOLOGY LETTERS, 132 (3). pp. 203-208. ISSN 0378-1097.
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Abstract
Genes encoding particulate methane monooxygenase and ammonia monooxygenase share high sequence identity. Degenerate oligonucleotide primers were designed, based on regions of shared amino acid sequence between the 27-kDa polypeptides, which are believed to contain the active sites, of particulate methane monooxygenase and ammonia monooxygenase. A 525-bp internal DNA fragment of the genes encoding these polypeptides (pmoA and amoA) from a variety of methanotrophic and nitrifying bacteria was amplified by PCR, cloned and sequenced. Representatives of each of the phylogenetic groups of both methanotrophs (alpha- and gamma-Proteobacteria) and ammonia-oxidizing nitrifying bacteria (beta-and gamma-Proteobacteria) were included, Analysis of the predicted amino acid sequences of these genes revealed strong conservation of both primary and secondary structure. Nitrosococcus oceanus AmoA showed higher identity to PmoA sequences from other members of the gamma-Proteobacteria than to AmoA sequences. These results suggest that the particulate methane monooxygenase and ammonia monooxygenase are evolutionarily related enzymes despite their different physiological roles in these bacteria.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | FEMS MICROBIOLOGY LETTERS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0378-1097 | ||||
Official Date: | 15 October 1995 | ||||
Dates: |
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Volume: | 132 | ||||
Number: | 3 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 203-208 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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