The Library
CORRELATION BETWEEN THE ACTIVITIES OF 5 RIBOSOME-INACTIVATING PROTEINS IN DEPURINATION OF TOBACCO RIBOSOMES AND INHIBITION OF TOBACCO MOSAIC-VIRUS INFECTION
Tools
UNSPECIFIED (1994) CORRELATION BETWEEN THE ACTIVITIES OF 5 RIBOSOME-INACTIVATING PROTEINS IN DEPURINATION OF TOBACCO RIBOSOMES AND INHIBITION OF TOBACCO MOSAIC-VIRUS INFECTION. PLANT JOURNAL, 5 (6). pp. 827-835. ISSN 0960-7412.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Abstract
The rRNA depurination activities of five ribosome-inactivating proteins (RIPs) were compared in vitro using yeast and tobacco leaf ribosomes as substrates. All of the RIPs (pokeweed antiviral protein (PAP), dianthin 32, tritin, barley RIP and ricin A-chain) were active on yeast ribosomes. PAP and dianthin 32 were highly active and ricin A-chain weakly active on tobacco ribosomes, whereas tritin and barley RIP were inactive. PAP and dianthin 32 were highly effective in inhibiting the formation of local lesions caused by tobacco mosaic virus (TMV) on tobacco leaves, whereas tritin, barley RIP and ricin A-chain were ineffective. The apparent anomaly between the in vitro rRNA depurination activity, but lack of antiviral activity of ricin A-chain was further investigated by assaying for rRNA depurination in situ following the topical application of the RIP to leaves. No activity was detected, a finding consistent with the apparent lack of antiviral activity of this RIP. Thus, it is concluded that there is a positive correlation between RIP-catalysed depurination of tobacco ribosomes and antiviral activity which gives strong support to the hypothesis that the antiviral activity of RIPs works through ribosome inactivation.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | S Agriculture > SB Plant culture | ||||
Journal or Publication Title: | PLANT JOURNAL | ||||
Publisher: | BLACKWELL SCIENCE LTD | ||||
ISSN: | 0960-7412 | ||||
Official Date: | June 1994 | ||||
Dates: |
|
||||
Volume: | 5 | ||||
Number: | 6 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 827-835 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |