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ELECTRON IONIZATION TANDEM MASS-SPECTROMETRY OF GLYCOSPHINGOLIPIDS .2. THE IDENTIFICATION OF A CARBOHYDRATE SEQUENCE CORRESPONDING TO A NOVEL REPETITIVE BLOOD GROUP-A HEPTAGLYCOSYLCERAMIDE
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UNSPECIFIED (1993) ELECTRON IONIZATION TANDEM MASS-SPECTROMETRY OF GLYCOSPHINGOLIPIDS .2. THE IDENTIFICATION OF A CARBOHYDRATE SEQUENCE CORRESPONDING TO A NOVEL REPETITIVE BLOOD GROUP-A HEPTAGLYCOSYLCERAMIDE. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 7 (6). pp. 421-426. ISSN 0951-4198.
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Abstract
In a previous paper, the presence in human kidney vein tissue of a novel blood group A heptaglycosylceramide based on the type-3 carbohydrate chain GalNAcalpha1-3(Fucalpha1-2)Galbeta1-3GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4Glcbeta1-1 Ceramide, was suggested based on thin-layer immunostaining and electron ionization mass spectrometry. Ions corresponding to a structure containing two deoxyhexoses, two hexosamines and three hexoses were identified, but no information was obtained from mass spectrometry concerning the carbohydrate sequence.5 In the present paper, we report the identification of carbohydrate sequence ions corresponding to a type-3 chain A heptaglycosylceramide by electron ionization-tandem mass spectrometry of a permethylated-reduced glycosphingolipid mixture isolated from human kidney vein tissue. The use of a microchannel-plate-array detector increased the sensitivity for collision-induced dissociation spectra by a factor of at least ten over a conventional electron multiplier.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QC Physics |
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Journal or Publication Title: | RAPID COMMUNICATIONS IN MASS SPECTROMETRY | ||||
Publisher: | JOHN WILEY & SONS LTD | ||||
ISSN: | 0951-4198 | ||||
Official Date: | June 1993 | ||||
Dates: |
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Volume: | 7 | ||||
Number: | 6 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 421-426 | ||||
Publication Status: | Published |
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