The Library
In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots
Tools
Gattolin, Stefano, Sorieul, Mathias, Hunter, P. R. (Paul R.), Khonsari, Roman H. and Frigerio, Lorenzo (2009) In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots. BMC Plant Biology, Vol.9 . Article 133. doi:10.1186/1471-2229-9-133 ISSN 1471-2229.
|
PDF
WRAP_Frigerio_In_Vivo.pdf - Requires a PDF viewer. Download (2249Kb) |
Official URL: http://dx.doi.org/10.1186/1471-2229-9-133
Abstract
Background: Tonoplast intrinsic proteins (TIPs) are widely used as markers for vacuolar
compartments in higher plants. Ten TIP isoforms are encoded by the Arabidopsis genome. For
several isoforms, the tissue and cell specific pattern of expression are not known.
Results: We generated fluorescent protein fusions to the genomic sequences of all members of
the Arabidopsis TIP family whose expression is predicted to occur in root tissues (TIP1;1 and 1;2;
TIP2;1, 2;2 and 2;3; TIP4;1) and expressed these fusions, both individually and in selected pairwise
combinations, in transgenic Arabidopsis. Analysis by confocal microscopy revealed that TIP
distribution varied between different cell layers within the root axis, with extensive co-expression
of some TIPs and more restricted expression patterns for other isoforms. TIP isoforms whose
expression overlapped appeared to localise to the tonoplast of the central vacuole, vacuolar bulbs
and smaller, uncharacterised structures.
Conclusion: We have produced a comprehensive atlas of TIP expression in Arabidopsis roots,
which reveals novel expression patterns for not previously studied TIPs.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QK Botany | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Library of Congress Subject Headings (LCSH): | Arabidopsis thaliana -- Genetics, Tonoplasts -- Research, Plant proteins, Roots (Botany) -- Physiology, Confocal microscopy | ||||
Journal or Publication Title: | BMC Plant Biology | ||||
Publisher: | BioMed Central Ltd. | ||||
ISSN: | 1471-2229 | ||||
Official Date: | 18 November 2009 | ||||
Dates: |
|
||||
Volume: | Vol.9 | ||||
Page Range: | Article 133 | ||||
DOI: | 10.1186/1471-2229-9-133 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | Leverhulme Trust (LT), European Union (EU) | ||||
Grant number: | LSH-2002-1.2.5-2 (EU), F/00215/AP (LT) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year