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A comparison of labeling and label-free mass spectrometry-based proteomics approaches
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Patel, Vibhuti J., Thalassinos, Konstantinos, Slade, Susan E., Connolly, Joanne B., Crombie, Andrew, Murrell, J. C. (J. Colin) and Scrivens, James H. (2009) A comparison of labeling and label-free mass spectrometry-based proteomics approaches. Journal of Proteome Research, Vol.8 (No.7). pp. 3752-3759. doi:10.1021/pr900080y ISSN 1535-3893.
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Official URL: http://dx.doi.org/10.1021/pr900080y
Abstract
The proteome of the recently discovered bacterium Methyiocella silvestris has been characterized using three profiling and comparative proteomics approaches. The organism has been grown on two different substrates enabling variations in protein expression to be identified. The results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. The sample preparation, instrumental time and sample loading requirements of the differing experiments are compared and discussed. A preliminary screen of the protein regulation results for biological significance has also been performed.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Journal or Publication Title: | Journal of Proteome Research | ||||
Publisher: | American Chemical Society | ||||
ISSN: | 1535-3893 | ||||
Official Date: | July 2009 | ||||
Dates: |
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Volume: | Vol.8 | ||||
Number: | No.7 | ||||
Number of Pages: | 8 | ||||
Page Range: | pp. 3752-3759 | ||||
DOI: | 10.1021/pr900080y | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Biological Mass Spectrometry and Protcomics Facility, Department of Biological Sciences, Warwick |
Data sourced from Thomson Reuters' Web of Knowledge
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