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Hb Belleville [10(A7)AlaThr] affects the determination of Hb A1c by routine cation exchange high performance liquid chromatography
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Williams, Jonathan P., Jackson, Hannah and Green, Brian N. (2009) Hb Belleville [10(A7)AlaThr] affects the determination of Hb A1c by routine cation exchange high performance liquid chromatography. Hemoglobin, Vol.33 (No.1). pp. 45-50. doi:10.1080/03630260802625915 ISSN 0363-0269.
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Official URL: http://dx.doi.org/10.1080/03630260802625915
Abstract
When analyzed by routine cation exchange high performance liquid chromatography (HPLC), the Hb A1c peak of a Caucasian diabetic patient from Yorkshire, UK, appeared to be an incompletely resolved doublet. One component (5.5%) eluted at the normal time for Hb A1c, whereas the other component (6.6%) eluted slightly later. The HPLC trace was otherwise normal. Analysis of the diabetic patient's blood and a tryptic digest thereof by electrospray ionization mass spectrometry (ESI-MS) identified the Hb Belleville trait. To relate Hb A1c determined by HPLC to and chain glycation determined by ESI-MS, several normal blood samples (5-14% Hb A1c) were analyzed by both techniques. The Hb A1c levels derived from the and chain glycation levels of the diabetic patient (12.9 and 12.4%, respectively) agreed with the sum of the two peaks (12.1%) in the HPLC trace. Similarly, Hb Belleville was detected and identified in another Caucasian, also from Yorkshire, with normal Hb A1c.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry R Medicine > RB Pathology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Journal or Publication Title: | Hemoglobin | ||||
Publisher: | Informa Healthcare | ||||
ISSN: | 0363-0269 | ||||
Official Date: | 2009 | ||||
Dates: |
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Volume: | Vol.33 | ||||
Number: | No.1 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 45-50 | ||||
DOI: | 10.1080/03630260802625915 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Data sourced from Thomson Reuters' Web of Knowledge
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