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Quantitative visualization of passive transport across bilayer lipid membranes
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Grime, John M. A., Edwards, Martin A., Rudd, Nicola C. and Unwin, Patrick R. (2008) Quantitative visualization of passive transport across bilayer lipid membranes. Proceedings of the National Academy of Sciences of the United States of America, Vol.105 (No.38). pp. 14277-14282. doi:10.1073/pnas.0803720105 ISSN 0027-8424.
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Official URL: http://dx.doi.org/10.1073/pnas.0803720105
Abstract
The ability to predict and interpret membrane permeation coefficients is of critical importance, particularly because passive transport is crucial for the effective delivery of many pharmaceutical agents to intracellular targets. We present a method for the quantitative measurement of the permeation coefficients of protonophores by using laser confocal scanning microscopy coupled to microelectrochemistry, which is amenable to precise modeling with the finite element method. The technique delivers well defined and high mass transport rates and allows rapid visualization of the entire pH distribution on both the cis and trans side of model bilayer lipid membranes (BLMs). A homologous series of carboxylic acids was investigated as probe molecules for BLMs composed of soybean phosphatidylcholine. Significantly, the permeation coefficient decreased with acyl tail length contrary to previous work and to Overton's rule. The reasons for this difference are considered, and we suggest that the applicability of Overton's rule requires re-evaluation.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QC Physics Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Ultramicroelectrodes, Confocal microscopy, Finite element method, Bilayer lipid membranes, Biological transport | ||||
Journal or Publication Title: | Proceedings of the National Academy of Sciences of the United States of America | ||||
Publisher: | National Academy of Sciences | ||||
ISSN: | 0027-8424 | ||||
Official Date: | 23 September 2008 | ||||
Dates: |
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Volume: | Vol.105 | ||||
Number: | No.38 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 14277-14282 | ||||
DOI: | 10.1073/pnas.0803720105 | ||||
Status: | Not Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), University of Warwick |
Data sourced from Thomson Reuters' Web of Knowledge
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