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The binding of auxin to the Arabidopsis auxin influx transporter AUX1
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Carrier, David J., Abu Bakar, Norliza Tendot, Swarup, Ranjan, Callaghan, Richard, Napier, R. (Richard), Bennett, Malcolm J. and Kerr, Ian D. (2008) The binding of auxin to the Arabidopsis auxin influx transporter AUX1. Plant Physiology, Volume 148 (Number 1). pp. 529-535. doi:10.1104/pp.108.122044 ISSN 0032-0889.
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Official URL: http://dx.doi.org/10.1104/pp.108.122044
Abstract
The cellular import of the hormone auxin is a fundamental requirement for the generation of auxin gradients that control a multitude of plant developmental processes. The AUX/LAX family of auxin importers, exemplified by AUX1 from Arabidopsis (Arabidopsis thaliana), has been shown to mediate auxin import when expressed heterologously. The quantitative nature of the interaction between AUX1 and its transport substrate indole-3-acetic acid (IAA) is incompletely understood, and we sought to address this in the present investigation. We expressed AUX1 to high levels in a baculovirus expression system and prepared membrane fragments from baculovirus-infected insect cells. These membranes proved suitable for determination of the binding of IAA to AUX1 and enabled us to determine a K-d of 2.6 mu M, comparable with estimates for the K m for IAA transport. The efficacy of a number of auxin analogues and auxin transport inhibitors to displace IAA binding from AUX1 has also been determined and can be rationalized in terms of their physiological effects. Determination of the parameters describing the initial interaction between a plant transporter and its hormone ligand provides novel quantitative data for modeling auxin fluxes.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QK Botany | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
Library of Congress Subject Headings (LCSH): | Auxin, Arabidopsis thaliana, Biological transport | ||||
Journal or Publication Title: | Plant Physiology | ||||
Publisher: | American Society of Plant Biologists | ||||
ISSN: | 0032-0889 | ||||
Official Date: | September 2008 | ||||
Dates: |
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Volume: | Volume 148 | ||||
Number: | Number 1 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 529-535 | ||||
DOI: | 10.1104/pp.108.122044 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 14 December 2015 | ||||
Date of first compliant Open Access: | 14 December 2015 | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Wellcome Trust (London, England), University of Nottingham , Malaysia | ||||
Grant number: | BB/C514958/1 (BBSRC), 077212/Z/05/Z (WT) |
Data sourced from Thomson Reuters' Web of Knowledge
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