The Library
The efficiency of Xenopus primordial germ cell migration depends on the germplasm mRNA encoding the PDZ domain protein Grip2
Tools
Kirilenko, Pavel, Weierud, Frida K., Zorn, Aaron M. and Woodland, Hugh R. (2008) The efficiency of Xenopus primordial germ cell migration depends on the germplasm mRNA encoding the PDZ domain protein Grip2. Differentiation, Volume 76 (Number 4). pp. 392-403. doi:10.1111/j.1432-0436.2007.00229.x ISSN 0301-4681.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1111/j.1432-0436.2007.00229.x
Abstract
A microarray analysis of vegetal pole sequences in the egg and early Xenopus laevis embryo identified Unigene Xl.14891 as a vegetally localized RNA. Analysis of the Xenopus tropicalis genome showed this Unigene to be localized near the 3' end of the Grip2 (glutamate receptor interacting protein 2) transcription unit. RACE showed that the Unigene represented the 3' UTR of Grip2 mRNA. Grip2 mRNA is present in the mitochondrial cloud of late pre-vitellogenic oocytes and then in the germplasm through oogenesis and early development until tailbud tadpole stages. Interference with Grip2 mRNA translation using two antisense morpholino oligos (MOs) impairs primordial germ cell (PGC) migration to the germinal ridges. Both MOs also inhibit swimming movements of the tailbud tadpole, known to involve glutamate receptors. We conclude that Grip2 has several functions in the embryo, including enabling efficient PGC migration.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QL Zoology |
||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Library of Congress Subject Headings (LCSH): | Xenopus, Germ cells, Germplasm resources, Cell migration, Messenger RNA | ||||
Journal or Publication Title: | Differentiation | ||||
Publisher: | Elsevier Ltd. | ||||
ISSN: | 0301-4681 | ||||
Official Date: | April 2008 | ||||
Dates: |
|
||||
Volume: | Volume 76 | ||||
Number: | Number 4 | ||||
Number of Pages: | 12 | ||||
Page Range: | pp. 392-403 | ||||
DOI: | 10.1111/j.1432-0436.2007.00229.x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Wellcome Trust (London, England), National Institutes of Health (U.S.) (NIH) | ||||
Grant number: | HD42572 (NIH) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |