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Influence of c-Myc and bcl-2 CO-expression on proliferation, apoptosis and survival in CHO cultures
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Ifandi, Vasiliki and Al-Rubeai, Mohamed (2007) Influence of c-Myc and bcl-2 CO-expression on proliferation, apoptosis and survival in CHO cultures. In: 19th Meeting of the European-Society-for-Animal-Cell-Technology (ESACT), Harrogate, UK, 5-8 Jun 2005. Published in: Cell Technology for Cell Products : Proceedings of the 19th ESACT Meeting, Harrogate, UK, June 5-8, 2005, Volume 3 pp. 217-220. ISBN 9781402054754.
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Abstract
Under the constitutive control of c-Myc over-expression the CHO cultures showed an increase in growth rate and maximum cell number accompanied by a similar decrease in specific glucose consumption rate. Additionally overexpression of c-Myc appeared to induce morphological transformation and partial anchorage-independence. Although, the c-myc transfected cell line exhibited apoptosis at much lower rates than it is widely reported and associated with the over-expression of c-Myc, it was nevertheless apparent that c-Myc was responsible for the induction of higher apoptotic rates when compared with the control cell line. Hence, the anti-apoptotic gene bcl-2 was also used to transfect the c-Myc CHO cell line, in order to reduce cell death. Over-expression of both oncoproteins resulted in a cell line that exhibited higher proliferation rates and maximum cell numbers, with a decrease in apoptosis when compared to the parental cell line.
Item Type: | Conference Item (Paper) | ||||
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Subjects: | T Technology > TP Chemical technology Q Science > QH Natural history > QH301 Biology |
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Journal or Publication Title: | Cell Technology for Cell Products : Proceedings of the 19th ESACT Meeting, Harrogate, UK, June 5-8, 2005 | ||||
Publisher: | Springer | ||||
ISBN: | 9781402054754 | ||||
Editor: | Smith, Rodney | ||||
Official Date: | 2007 | ||||
Dates: |
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Volume: | Volume 3 | ||||
Number of Pages: | 4 | ||||
Page Range: | pp. 217-220 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Conference Paper Type: | Paper | ||||
Title of Event: | 19th Meeting of the European-Society-for-Animal-Cell-Technology (ESACT) | ||||
Type of Event: | Conference | ||||
Location of Event: | Harrogate, UK | ||||
Date(s) of Event: | 5-8 Jun 2005 | ||||
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