The Library
Niobium uptake and release by bacterial ferric ion binding protein
Tools
Shi, Yanbo, Harvey, Ian, Campopiano, Dominic and Sadler, P. J. (2010) Niobium uptake and release by bacterial ferric ion binding protein. Bioinorganic Chemistry and Applications . doi:10.1155/2010/307578 ISSN 1565-3633.
PDF
WRAP_Sadler_niobium_uptake.pdf - Requires a PDF viewer. Download (1178Kb) |
Official URL: http://dx.doi.org/10.1155/2010/307578
Abstract
Ferric ion binding proteins (Fbps) transport FeIII across the periplasm and are vital for the virulence of many Gram negative
bacteria. Iron(III) is tightly bound in a hinged binding cleft with octahedral coordination geometry involving binding to protein
side chains (including tyrosinate residues) together with a synergistic anion such as phosphate. Niobium compounds are of
interest for their potential biological activity, which has been little explored. We have studied the binding of cyclopentadienyl
and nitrilotriacetato NbV complexes to the Fbp from Neisseria gonorrhoeae by UV-vis spectroscopy, chromatography, ICP-OES,
mass spectrometry, and Nb K-edge X-ray absorption spectroscopy. These data suggest that NbV binds strongly to Fbp and that a
dinuclear NbV centre can be readily accommodated in the interdomain binding cleft. The possibility of designing niobium-based
antibiotics which block iron uptake by pathogenic bacteria is discussed.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Iron proteins, Protein binding, Gram-negative bacteria, Niobium, Antibiotics -- Research | ||||
Journal or Publication Title: | Bioinorganic Chemistry and Applications | ||||
Publisher: | Hindawi Publishing Corporation | ||||
ISSN: | 1565-3633 | ||||
Official Date: | 2010 | ||||
Dates: |
|
||||
DOI: | 10.1155/2010/307578 | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Wellcome Trust (London, England), Edinburgh Protein Interaction Centre |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year