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Novel use of stir bar sorptive extraction (SBSE) as a tool for isolation of oviposition site attractants for gravid Culex quinquefasciatus
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Carson, Connor, Birkett, Michael Alexander, Logan, J. G., Mawa, K., Pates, H. V., Pickett, J. A. (John A.), Rwegoshora, R. T., Tungu, P. K. and Cameron, M. M. (2010) Novel use of stir bar sorptive extraction (SBSE) as a tool for isolation of oviposition site attractants for gravid Culex quinquefasciatus. Bulletin of Entomological Research, Vol.100 (No.1). pp. 1-7. doi:10.1017/S0007485309006701 ISSN 0007-4853.
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Official URL: http://dx.doi.org/10.1017/S0007485309006701
Abstract
Mosquitoes such as Culex quinquefasciatus Say (Diptera: Culicidae) are important vectors of organisms that cause disease in humans. Research into the development of effective standardized odour baits for blood-fed females (oviposition attractants), to enable entomological monitoring of vector populations, is hampered by complex protocols for extraction of physiologically active volatile chemicals from natural breeding site water samples, which have produced inconsistent results. Air entrainment and solvent extraction are technically demanding methods and are impractical for use in resource poor environments where mosquito-borne disease is most prevalent. This study reports the first use of a simple, robust extraction technique, stir bar sorptive extraction (SBSE), to extract behaviourally active small lipophilic molecules (SLMs) present in water samples collected from Cx. quinquefasciatus breeding sites in Tanzania. Extracts from a pit latrine and from a cess pool breeding site attracted more gravid Cx. quinquefasciatus in pair choice bioassays than control extracts, and coupled gas chromatography-electroantennography (GC-EAG) allowed tentative identification of 15 electrophysiologically active chemicals, including the known oviposition attractant, skatole (3-methylindole). Here, we have demonstrated, using simple pair choice bioassays in controlled laboratory conditions, that SBSE is effective for the extraction of behaviourally and electrophysiologically active semiochemicals from mosquito breeding site waters. Further research is required to confirm that SBSE is an appropriate technique for use in field surveys in the search for oviposition cues for Cx. quinquefasciatus.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QL Zoology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Library of Congress Subject Headings (LCSH): | Culex quinquefasciatus -- Research, Communicable diseases -- Transmission, Insect baits and repellents, Semiochemicals | ||||
Journal or Publication Title: | Bulletin of Entomological Research | ||||
Publisher: | Cambridge University Press | ||||
ISSN: | 0007-4853 | ||||
Official Date: | 2010 | ||||
Dates: |
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Volume: | Vol.100 | ||||
Number: | No.1 | ||||
Page Range: | pp. 1-7 | ||||
DOI: | 10.1017/S0007485309006701 | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) |
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