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Diffusion of proteins in chloroplast membranes
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Vladimirou, Elina (2010) Diffusion of proteins in chloroplast membranes. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b2487908~S15
Abstract
The lateral diffusion of Hcf106, a core subunit of the ΔpH-dependent translocon
in higher plants, and chlorophyll-containing complexes is thoroughly investigated
by Fluorescent Recovery After Photobleaching (FRAP) and Fluorescent
Loss in Photobleaching (FLIP). Simple models of a thylakoid network are derived
directly from confocal fluorescence microscopy images by Monte Carlo optimisation.
These networks are used as the domain for diffusion simulations using the
Particle Strength Exchange method, allowing determination of diffusion coefficients for the thylakoid protein Hcf106 and for chlorophyll-containing complexes.
Extending the mobility studies to the chloroplast outer membrane, diffusion coefficients
are estimated for Toc159, a component of the chloroplast outer membrane
translocon TOC.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QK Botany | ||||
Library of Congress Subject Headings (LCSH): | Chloroplast membranes, Plant proteins -- Physiological transport | ||||
Official Date: | September 2010 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | Molecular Organisation and Assembly in Cells | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Robinson, Colin, 1958- ; Kirkilionis, Markus, 1962- | ||||
Sponsors: | Engineering and Physical Sciences Research Council (EPSRC) | ||||
Extent: | xii, 169 p. : ill., charts | ||||
Language: | eng |
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