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Selective inhibition of carotenoid cleavage dioxygenases : phenotypic effects on shoot branching
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Sergeant, Martin J., Li, J.J., Fox, C., Brookbank, N., Rea, Dean, Bugg, Tim and Thompson, Andrew J. (2009) Selective inhibition of carotenoid cleavage dioxygenases : phenotypic effects on shoot branching. Journal of Biological Chemistry, Vol.284 (No.8). pp. 5257-5264. doi:10.1074/jbc.M805453200 ISSN 0021-9258.
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Official URL: http://dx.doi.org/10.1074/jbc.M805453200
Abstract
Members of the carotenoid cleavage dioxygenase family catalyse the oxidative cleavage of carotenoids at various chain positions, leading to the formation of a wide range of apocarotenoid signalling molecules. To explore the functions of this diverse enzyme family, we have used a chemical genetic approach to design selective inhibitors for different classes of carotenoid cleavage dioxygenase. A set of 18 arylalkyl-hydroxamic acids was synthesised in which the distance between an iron-chelating hydroxamic acid and an aromatic ring was varied; these compounds were screened as inhibitors of four different enzyme classes, either in vitro or in vivo. Potent inhibitors were found that selectively inhibited enzymes that cleave carotenoids at the 9,10 position; 50% inhibition was achieved at sub-micromolar concentrations. Application of certain inhibitors at 100 microM to Arabidopsis node explants or whole plants led to increased shoot branching, consistent with inhibition of 9,10-cleavage.
Item Type: | Journal Article | ||||
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Subjects: | S Agriculture > SB Plant culture | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
Library of Congress Subject Headings (LCSH): | Carotenoids | ||||
Journal or Publication Title: | Journal of Biological Chemistry | ||||
Publisher: | American Society for Biochemistry and Molecular Biology | ||||
ISSN: | 0021-9258 | ||||
Official Date: | 20 February 2009 | ||||
Dates: |
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Volume: | Vol.284 | ||||
Number: | No.8 | ||||
Page Range: | pp. 5257-5264 | ||||
DOI: | 10.1074/jbc.M805453200 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
Grant number: | BB/D005787 (BBSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
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