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Novel biphasic role of lipoxinA4 on expression of cyclooxygenase-2 in lipopolysaccharide-stimulated lung fibroblasts
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Zheng, Shengxing, Wang, Qian, He, Qian, Song, Xiaorong, Ye, Duyun, Smith, F. Gao (Fang Gao), Jin, Shengwei and Lian, QingQuan (2011) Novel biphasic role of lipoxinA4 on expression of cyclooxygenase-2 in lipopolysaccharide-stimulated lung fibroblasts. Mediators of Inflammation, Vol.2011 (No.745340). doi:10.1155/2011/745340 ISSN 1466-1861.
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Official URL: http://dx.doi.org/10.1155/2011/745340
Abstract
Fibroblasts are important to host defence and immunity, can also as initiators of inflammation as well. As the endogenous
"braking signal", Lipoxins can regulate anti-inflammation and the resolution of inflammation. We investigated the effect of
lipoxinA4 on the expression of cyclooxygenase-2 in lipopolysaccharide-stimulated lung fibroblasts. We demonstrated that the
expression of cyclooxygenase-2 protein was significantly increased and peaked initially at 6 hours, with a second increase,
with maximal levels occurring 24 hours after lipopolysaccharide challenge. ProstaglandinE2 levels also peaked at 6 hours, and
prostaglandinD2 levels were increased at both 6 and 24 hours. Exogenous lipoxinA4 inhibited the first peak of cyclooxygenase-2
expression as well as the production of prostaglandinE2 induced by lipopolysaccharide in a dose-dependent manner. In contrast,
exogenous lipoxinA4 increased the second peak of cyclooxygenase-2 expression as well as the production of prostaglandinD2
induced by lipopolysaccharide in a dose-dependent manner. LipoxinA4 receptor mRNA expression was markedly stimulated by
lipopolysaccharide but inhibited by lipoxinA4. We present evidence for a novel biphasic role of lipoxinA4 on the expression of
cyclooxygenase-2 in lipopolysaccharide-stimulated lung fibroblasts, whereby LXA4 has an anti-inflammatory and proresolving
activity in lung fibroblasts following LPS stimulation.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QP Physiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
Library of Congress Subject Headings (LCSH): | Lipoxins, Fibroblasts, Cyclooxygenase 2 | ||||
Journal or Publication Title: | Mediators of Inflammation | ||||
Publisher: | Hindawi Publishing Corporation | ||||
ISSN: | 1466-1861 | ||||
Official Date: | 2011 | ||||
Dates: |
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Volume: | Vol.2011 | ||||
Number: | No.745340 | ||||
Number of Pages: | 9 | ||||
DOI: | 10.1155/2011/745340 | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 17 December 2015 | ||||
Date of first compliant Open Access: | 17 December 2015 | ||||
Funder: | Guo jia zi ran ke xue ji jin wei yuan hui (China) [National Natural Science Foundation of China] (NSFC), Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents | ||||
Grant number: | 30772088 (NSFC), 81070061 (NSFC) |
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