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Seed storage oil mobilization is important but not essential for germination or seedling establishment in Arabidopsis
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Kelly, Amélie A., Quettier, Anne-Laure, Shaw, Eve and Eastmond, Peter J. (2011) Seed storage oil mobilization is important but not essential for germination or seedling establishment in Arabidopsis. Plant Physiology, Vol.157 (No.2). pp. 866-875. doi:10.1104/pp.111.181784 ISSN 0032-0889.
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Official URL: http://dx.doi.org/10.1104/pp.111.181784
Abstract
Triacylglycerol (TAG) is a major storage reserve in many plant seeds. We previously identified a TAG lipase mutant called
sugar-dependent1 (sdp1) that is impaired in TAG hydrolysis following Arabidopsis (Arabidopsis thaliana) seed germination
(Eastmond, 2006). The aim of this study was to identify additional lipases that account for the residual TAG hydrolysis
observed in sdp1. Mutants were isolated in three candidate genes (SDP1-LIKE [SDP1L], ADIPOSE TRIGLYCERIDE LIPASELIKE,
and COMPARATIVE GENE IDENTIFIER-58-LIKE). Analysis of double, triple, and quadruple mutants showed that
SDP1L is responsible for virtually all of the residual TAG hydrolysis present in sdp1 seedlings. Oil body membranes purified
from sdp1 sdp1L seedlings were deficient in TAG lipase activity but could still hydrolyze di- and monoacylglycerol. SDP1L is
expressed less strongly than SDP1 in seedlings. However, SDP1L could partially rescue TAG breakdown in sdp1 seedlings
when expressed under the control of the SDP1 or 35S promoters and in vitro assays showed that both SDP1 and SDP1L can
hydrolyze TAG, in preference to diacylglycerol or monoacylglycerol. Seed germination was slowed in sdp1 sdp1L and
postgerminative seedling growth was severely retarded. The frequency of seedling establishment was also reduced, but sdp1
sdp1L was not seedling lethal under normal laboratory growth conditions. Our data show that together SDP1 and SDP1L
account for at least 95% of the rate of TAG hydrolysis in Arabidopsis seeds, and that this hydrolysis is important but not
essential for seed germination or seedling establishment.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QK Botany | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Arabidopsis -- Genetics, Arabidopsis -- Seeds, Germination -- Physiological aspects | ||||
Journal or Publication Title: | Plant Physiology | ||||
Publisher: | American Society of Plant Biologists | ||||
ISSN: | 0032-0889 | ||||
Official Date: | August 2011 | ||||
Dates: |
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Volume: | Vol.157 | ||||
Number: | No.2 | ||||
Page Range: | pp. 866-875 | ||||
DOI: | 10.1104/pp.111.181784 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 18 December 2015 | ||||
Date of first compliant Open Access: | 18 December 2015 | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
Grant number: | BB/E022197/1 (BBSRC) |
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