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Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart
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Inagawa, T., Yamada-Inagawa, T., Eydmann, T., Mian, I. S., Wang, T. S. and Dalgaard, Jacob Z. (2009) Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart. Proceedings of the National Academy of Sciences of the United States of America, Vol.106 (No.19). pp. 7927-7932. doi:10.1073/pnas.0812323106 ISSN 0027-8424.
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Official URL: http://dx.doi.org/10.1073/pnas.0812323106
Abstract
Here, we identify a phylogenetically conserved Schizosaccharomyces pombe factor, named Rtf2, as a key requirement for efficient replication termination at the site-specific replication barrier RTS1. We show that Rtf2, a proliferating cell nuclear antigen-interacting protein, promotes termination at RTS1 by preventing replication restart; in the absence of Rtf2, we observe the establishment of “slow-moving” Srs2-dependent replication forks. Analysis of the pmt3 (SUMO) and rtf2 mutants establishes that pmt3 causes a reduction in RTS1 barrier activity, that rtf2 and pmt3 are nonadditive, and that pmt3 (SUMO) partly suppresses the rtf2-dependent replication restart. Our results are consistent with a model in which Rtf2 stabilizes the replication fork stalled at RTS1 until completion of DNA synthesis by a converging replication fork initiated at a flanking origin.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Proceedings of the National Academy of Sciences of the United States of America | ||||
Publisher: | National Academy of Sciences | ||||
ISSN: | 0027-8424 | ||||
Official Date: | 12 May 2009 | ||||
Dates: |
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Volume: | Vol.106 | ||||
Number: | No.19 | ||||
Page Range: | pp. 7927-7932 | ||||
DOI: | 10.1073/pnas.0812323106 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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