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Short term effects of c-MYC activation on β-cell physiology and glucose homeostasis in the pIns-c-MycERTAM transgenic mouse
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Wang, Yi-Fang (2012) Short term effects of c-MYC activation on β-cell physiology and glucose homeostasis in the pIns-c-MycERTAM transgenic mouse. PhD thesis, University of Warwick.
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WRAP_THESIS_Wang_2012.pdf - Submitted Version Download (9Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b2565708~S1
Abstract
Injection of 4-hydroxytamoxifen (4-OHT) activates the oncogene c-myc in
transgenic pIns-c-MycERTAM mice, triggering β-cell proliferation in the short term as
well as apoptosis and reduced insulin secretion, leading to hyperglycaemia. This
hyperglycaemia is preceded by a short period of hypoglycaemia, which may be
caused by: (i) increased insulin secretion or release from dying cells; (ii) rapid β-cell
proliferation; and (iii) increased insulin sensitivity. This thesis characterizes the
initial stages of the expression of c-MYC in the plns-c-MycERTAM mouse model and
attempts to identify the causes of the transient hypoglycaemia using mathematical
models. Furthermore, microarray data were analysed to investigate the early
hypoglycaemia from the point of view of transcriptomics. The size and mass of β-cells were quantified during the transient period of hypoglycaemia by means of
immunohistochemistry. These data were incorporated in a detailed mathematical
model of β-cell dynamics.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QL Zoology | ||||
Library of Congress Subject Headings (LCSH): | Mice -- Physiology, Mice -- Genetics, Hypoglycemia, Pancreatic beta cells | ||||
Official Date: | January 2012 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Khan, Michael ; Epstein, D. B. A. | ||||
Extent: | 192, [11] leaves : ill., charts | ||||
Language: | eng |
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