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Ferric ion (hydr)oxo clusters in the “Venus flytrap” cleft of FbpA : Mössbauer, calorimetric and mass spectrometric studies
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Mukherjee, Arindam, Bilton, Paul R., Mackay, Logan, Janoschka, Adam, Zhu, Haizhong, Rea, Dean, Langridge-Smith, Pat R. R., Campopiano, Dominic J., Teschner, Thomas, Trautwein, Alfred, Schünemann, Volker and Sadler, P. J. (2012) Ferric ion (hydr)oxo clusters in the “Venus flytrap” cleft of FbpA : Mössbauer, calorimetric and mass spectrometric studies. JBIC Journal of Biological Inorganic Chemistry, Vol.17 (No.4). pp. 573-588. doi:10.1007/s00775-012-0878-z ISSN 0949-8257.
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WRAP_Mukherjee_480_JBIC-11-05-00115_Script_Rev3.pdf - Accepted Version Download (1303Kb) | Preview |
Official URL: http://dx.doi.org/10.1007/s00775-012-0878-z
Abstract
Isothermal calorimetric studies of the binding of iron(III) citrate to ferric ion binding protein from Neisseria gonorrhoeae suggested the complexation of a tetranuclear iron(III) cluster as a single step binding event (apparent binding constant K appITC = 6.0(5) × 105 M−1). High-resolution Fourier transform ion cyclotron resonance mass spectrometric data supported the binding of a tetranuclear oxo(hydroxo) iron(III) cluster of formula [Fe4O2(OH)4(H2O)(cit)]+ in the interdomain binding cleft of FbpA. The mutant H9Y-nFbpA showed a twofold increase in the apparent binding constant [K appITC = 1.1(7) × 106 M−1] for the tetranuclear iron(III) cluster compared to the wild-type protein. Mössbauer spectra of Escherichia coli cells overexpressing FbpA and cultured in the presence of added 57Fe citrate were indicative of the presence of dinuclear and polynuclear clusters. FbpA therefore appears to have a strong affinity for iron clusters in iron-rich environments, a property which might endow the protein with new biological functions.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Carrier proteins, Mössbauer spectroscopy, Transferrin, Calorimetry, Metal clusters, Mass spectrometry, Iron ions | ||||
Journal or Publication Title: | JBIC Journal of Biological Inorganic Chemistry | ||||
Publisher: | Springer | ||||
ISSN: | 0949-8257 | ||||
Official Date: | April 2012 | ||||
Dates: |
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Volume: | Vol.17 | ||||
Number: | No.4 | ||||
Page Range: | pp. 573-588 | ||||
DOI: | 10.1007/s00775-012-0878-z | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 21 December 2015 | ||||
Date of first compliant Open Access: | 21 December 2015 | ||||
Funder: | European Commission (EC), Engineering and Physical Sciences Research Council (EPSRC), European Regional Development Fund (ERDF), Advantage West Midlands (AWM), European Research Council (ERC) | ||||
Grant number: | 247450 (ERC) |
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