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Five arabidopsis reticulon isoforms share endoplasmic reticulum location, topology, and membrane-shaping properties
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Sparkes, Imogen, Tolley, Nicholas, Aller, Isabel, Svozil, Julia, Osterrieder, Anne, Botchway, Stanley, Mueller, Christopher, Frigerio, Lorenzo and Hawes, C. R. (2010) Five arabidopsis reticulon isoforms share endoplasmic reticulum location, topology, and membrane-shaping properties. Plant Cell, Vol.22 (No.4). pp. 1333-1343. doi:10.1105/tpc.110.074385 ISSN 1040-4651.
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Official URL: http://dx.doi.org/10.1105/tpc.110.074385
Abstract
The cortical endoplasmic reticulum (ER) in tobacco (Nicotiana tabacum) epidermal cells is a network of tubules and cisternae undergoing dramatic rearrangements. Reticulons are integral membrane proteins involved in shaping ER tubules. Here, we characterized the localization, topology, effect, and interactions of five Arabidopsis thaliana reticulons (RTNs), isoforms 1-4 and 13, in the cortical ER. Our results indicate that RTNLB13 and RTNLB1-4 colocate to and constrict the tubular ER membrane. All five RTNs preferentially accumulate on ER tubules and are excluded from ER cisternae. All isoforms share the same transmembrane topology, with N and C termini facing the cytosol and four transmembrane domains. We show by Forster resonance energy transfer and fluorescence lifetime imaging microscopy that several RTNs have the capacity to interact with themselves and each other, and we suggest that oligomerization is responsible for their residence in the ER membrane. We also show that a complete reticulon homology domain is required for both RTN residence in high-curvature ER membranes and ER tubule constriction, yet it is not necessary for homotypic interactions.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry S Agriculture > SB Plant culture Q Science > QH Natural history > QH301 Biology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Journal or Publication Title: | Plant Cell | ||||
Publisher: | American Society of Plant Biologists | ||||
ISSN: | 1040-4651 | ||||
Official Date: | April 2010 | ||||
Dates: |
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Volume: | Vol.22 | ||||
Number: | No.4 | ||||
Number of Pages: | 11 | ||||
Page Range: | pp. 1333-1343 | ||||
DOI: | 10.1105/tpc.110.074385 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 3 December 2015 | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Leverhulme Trust (LT), Leverhulme, Heidelberg University, European Union | ||||
Grant number: | F/00 382/G, BB/F008147/1, LSH-2002-1.2.5-2, F/00215/AP |
Data sourced from Thomson Reuters' Web of Knowledge
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