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Stable isotope probing - linking microbial identity to function
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UNSPECIFIED (2005) Stable isotope probing - linking microbial identity to function. Nature, 3 (6). pp. 499-504. doi:10.1038/nrmicro1162
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Official URL: http://dx.doi.org/10.1038/nrmicro1162
Abstract
Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as C-13, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and C-13-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics.
Item Type: | Journal Item | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | Nature | ||||
Publisher: | NATURE PUBLISHING GROUP | ||||
ISSN: | 1740-1526 | ||||
Official Date: | June 2005 | ||||
Dates: |
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Volume: | 3 | ||||
Number: | 6 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 499-504 | ||||
DOI: | 10.1038/nrmicro1162 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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