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Spatially coordinated dynamic gene transcription in living pituitary tissue
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Featherstone, Katie, Hey, Kirsty L., Momiji, Hiroshi, McNamara, A. V., Patist, A. L., Woodburn, J., Spiller , D. G., Christian, H. C., McNeilly, A. S., Mullins, J. J. , Finkenstädt, Bärbel, Rand, D. A. (David A.), White, M. R. H. and David, J. R. E. (2016) Spatially coordinated dynamic gene transcription in living pituitary tissue. eLife, 5 . e08494. doi:10.7554/eLife.08494 ISSN 2050-084X.
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Official URL: http://dx.doi.org/10.7554/eLife.08494
Abstract
Transcription at individual genes in single cells is often pulsatile and stochastic. A key question emerges regarding how this behaviour contributes to tissue phenotype, but it has been a challenge to quantitatively analyse this in living cells over time, as opposed to studying snap-shots of gene expression state. We have used imaging of reporter gene expression to track transcription in living pituitary tissue. We integrated live-cell imaging data with statistical modelling for quantitative real-time estimation of the timing of switching between transcriptional states across a whole tissue. Multiple levels of transcription rate were identified, indicating that gene expression is not a simple binary ‘on-off’ process. Immature tissue displayed shorter durations of high-expressing states than the adult. In adult pituitary tissue, direct cell contacts involving gap junctions allowed local spatial coordination of prolactin gene expression. Our findings identify how heterogeneous transcriptional dynamics of single cells may contribute to overall tissue behaviour.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Statistics | ||||||
Library of Congress Subject Headings (LCSH): | Genetic transcription, Pituitary gland | ||||||
Journal or Publication Title: | eLife | ||||||
Publisher: | eLife Sciences Publications Ltd. | ||||||
ISSN: | 2050-084X | ||||||
Official Date: | 1 February 2016 | ||||||
Dates: |
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Volume: | 5 | ||||||
Article Number: | e08494 | ||||||
DOI: | 10.7554/eLife.08494 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Date of first compliant deposit: | 12 April 2017 | ||||||
Date of first compliant Open Access: | 12 April 2017 | ||||||
Funder: | Wellcome Trust (London, England), Engineering and Physical Sciences Research Council (EPSRC), Medical Research Council (Canada) (MRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||
Grant number: | WT091688 (London, England), ASTAA1112.KXH (EPSRC), MR/K015885/1 (MRC), BB/K003097/1 (BBSRC) |
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