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Glucocorticoid inhibition of granulocyte macrophage-colony-stimulating factor from T cells is independent of control by nuclear factor-kappa B and conserved lymphokine element 0
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UNSPECIFIED (2004) Glucocorticoid inhibition of granulocyte macrophage-colony-stimulating factor from T cells is independent of control by nuclear factor-kappa B and conserved lymphokine element 0. Journal of Molecular Biology, 30 (4). pp. 555-563. doi:10.1165/rcmb.2003-0295OC ISSN 1044-1549.
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Official URL: http://dx.doi.org/10.1165/rcmb.2003-0295OC
Abstract
Release of granulocyte macrophage-colony-stimulating factor (GM-CSF) from T cells is important in the differentiation, maturation, and survival of inflammatory cells. Here the induction of GMCSF expression from T cells was dependent on transcription and translation and was prevented by dexamethasone. In primary human CD3+ T cells, up to 3.3 kb of human GM-CSF promoter was strongly activated by PMA + PHA. Mutations in either the -85/-76 nuclear factor (NF)-kappaB site or the activator protein-1 region in the -54/-31 conserved lymphokine element 0 (CLEO) site substantially reduced promoter activity. Both GM-CSF promoter and NF-kappaB-dependent constructs were unresponsive to dexamethasone whereas the release of GM-CSF was potently repressed. Analysis of GM-CSF mRNA and protein expression at various time points and the effect of adding dexamethasone after the stimulus revealed the existence of potent mechanisms of inhibition acting at a translational level. The expression of tristetraproline and HuR, proteins that bind the AU-rich element in the GM-CSF 3'-untranslated region was unaffected by dexamethasone and overall AU-rich element binding activity was unaltered. Taken together our data support an important role for the NF-kappaB and CLEO sites in the transcriptional control of GM-CSF expression in primary human T cells and suggest that post-transcriptional/transiational mechanisms are key mediators of glucocorticoid-dependent repression.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology R Medicine > RC Internal medicine |
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Journal or Publication Title: | Journal of Molecular Biology | ||||
Publisher: | AMER THORACIC SOC | ||||
ISSN: | 1044-1549 | ||||
Official Date: | April 2004 | ||||
Dates: |
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Volume: | 30 | ||||
Number: | 4 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 555-563 | ||||
DOI: | 10.1165/rcmb.2003-0295OC | ||||
Publication Status: | Published |
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