The Library
Expression, purification and preliminary crystallographic analysis of phosphoribosyl isomerase (PriA) from Streptomyces coelicolor
Tools
UNSPECIFIED (2004) Expression, purification and preliminary crystallographic analysis of phosphoribosyl isomerase (PriA) from Streptomyces coelicolor. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 60 (Part 3). pp. 534-536. doi:10.1107/S0907444903028877 ISSN 0907-4449.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1107/S0907444903028877
Abstract
The priA gene encoding the enzyme phosphoribosyl isomerase from Streptomyces coelicolor, a novel bifunctional enzyme involved in both histidine and tryptophan biosynthesis, was heterologously expressed and purified in Escherichia coli as an N-terminal His-tag fusion. The purified recombinant enzyme was crystallized using the hanging-drop method in 1.50 M ammonium sulfate and 100 mM sodium citrate pH 4.8. Crystals were obtained of up to 0.05x0.05x0.3 mm in size. A full data set to 2 Angstrom resolution was collected at the ESRF beamline ID14-1 and space group P3(1,2)21 was assigned, with unit-cell parameters a=65.1, c=104.7 Angstrom.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
||||
Journal or Publication Title: | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | ||||
Publisher: | BLACKWELL MUNKSGAARD | ||||
ISSN: | 0907-4449 | ||||
Official Date: | March 2004 | ||||
Dates: |
|
||||
Volume: | 60 | ||||
Number: | Part 3 | ||||
Number of Pages: | 3 | ||||
Page Range: | pp. 534-536 | ||||
DOI: | 10.1107/S0907444903028877 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |