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The N-end rule pathway promotes seed germination and establishment through removal of ABA sensitivity in Arabidopsis
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Holman, Tara J., Jones, Peter D., Russell, Laurel, Medhurst, Ann, Úbeda-Tomás, Susanna, Talloji, Prabhavathi, Marquez, Julietta, Schmuths, Heike, Tung, Swee-Ang, Taylor , Ian B, Footitt, Steven, Bachmair, Andreas, Theodoulou, Frederica L. and Holdsworth, Michael J. (2009) The N-end rule pathway promotes seed germination and establishment through removal of ABA sensitivity in Arabidopsis. Proceedings of the National Academy of Sciences, Vol.10 (No.11). pp. 4549-4554. doi:10.1073/pnas.0810280106 ISSN 0027-8424.
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Official URL: http://dx.doi.org/10.1073/pnas.0810280106
Abstract
The N-end rule pathway targets protein degradation through the identity of the amino-terminal residue of specific protein substrates. Two components of this pathway in Arabidopsis thaliana, PROTEOLYSIS6 (PRT6) and arginyl-tRNA:protein arginyltransferase (ATE), were shown to regulate seed after-ripening, seedling sugar sensitivity, seedling lipid breakdown, and abscisic acid (ABA) sensitivity of germination. Sensitivity of prt6 mutant seeds to ABA inhibition of endosperm rupture reduced with after-ripening time, suggesting that seeds display a previously undescribed window of sensitivity to ABA. Reduced root growth of prt6 alleles and the ate1 ate2 double mutant was rescued by exogenous sucrose, and the breakdown of lipid bodies and seed-derived triacylglycerol was impaired in mutant seedlings, implicating the N-end rule pathway in control of seed oil mobilization. Epistasis analysis indicated that PRT6 control of germination and establishment, as exemplified by ABA and sugar sensitivity, as well as storage oil mobilization, occurs at least in part via transcription factors ABI3 and ABI5. The N-end rule pathway of protein turnover is therefore postulated to inactivate as-yet unidentified key component(s) of ABA signaling to influence the seed-to-seedling transition.
Item Type: | Journal Article | ||||
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Subjects: | S Agriculture > SB Plant culture | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
Library of Congress Subject Headings (LCSH): | Arabidopsis thaliana, Proteins -- Denaturation, Abscisic acid, Aminoacyl-tRNA, Transferases, Lipids -- Metabolism | ||||
Journal or Publication Title: | Proceedings of the National Academy of Sciences | ||||
Publisher: | National Academy of Sciences | ||||
ISSN: | 0027-8424 | ||||
Official Date: | 17 March 2009 | ||||
Dates: |
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Volume: | Vol.10 | ||||
Number: | No.11 | ||||
Page Range: | pp. 4549-4554 | ||||
DOI: | 10.1073/pnas.0810280106 | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Lawes Agricultural Trust (LAT), National Fruit and Cider Institute (Great Britain) (NFCI), University of Nottingham, Deutsche Forschungsgemeinschaft (DFG) | ||||
Grant number: | BA 1158/3/1 (DFG) |
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