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Crystallization and preliminary X-ray diffraction analysis of a dihaem cytochrome c peroxidase from Paracoccus denitrificans
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UNSPECIFIED (2004) Crystallization and preliminary X-ray diffraction analysis of a dihaem cytochrome c peroxidase from Paracoccus denitrificans. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 60 (Part 2). pp. 331-333. doi:10.1107/S0907444903026519 ISSN 0907-4449.
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Official URL: http://dx.doi.org/10.1107/S0907444903026519
Abstract
Cytochrome c peroxidase was isolated from Paracoccus denitrificans and purified to homogeneity in three steps prior to crystallization. Two different diffraction-quality crystal forms were obtained by the hanging-drop vapour-diffusion method using a number of screening conditions. The best (needle-shaped) crystal form is suitable for structural studies and was grown from solutions containing 20% PEG 8000, 0.1 MTris pH 8.5 and 0.2 M MgCl2. Crystals grew to a maximum length of approximately 0.7 mm and belong to the primitive monoclinic space group P2(1), with unit-cell parameters a = 78.3, b = 51.0, c=167.2 Angstrom, beta = 97.9degrees. After a dehydration step and extensive optimization of the cryocooling conditions, a complete data set was collected to 2.2 Angstrom from a native crystal of the fully oxidized form of the enzyme using synchrotron radiation.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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Journal or Publication Title: | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | ||||
Publisher: | BLACKWELL MUNKSGAARD | ||||
ISSN: | 0907-4449 | ||||
Official Date: | February 2004 | ||||
Dates: |
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Volume: | 60 | ||||
Number: | Part 2 | ||||
Number of Pages: | 3 | ||||
Page Range: | pp. 331-333 | ||||
DOI: | 10.1107/S0907444903026519 | ||||
Publication Status: | Published |
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