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Multivalent conjugates of poly-gamma-D-glutamic acid from Bacillus licheniformis with antibody F(ab ') and glycopeptide Ligands
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UNSPECIFIED (2003) Multivalent conjugates of poly-gamma-D-glutamic acid from Bacillus licheniformis with antibody F(ab ') and glycopeptide Ligands. BIOCONJUGATE CHEMISTRY, 14 (6). pp. 1148-1155. doi:10.1021/bc020019m ISSN 1043-1802.
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Official URL: http://dx.doi.org/10.1021/bc020019m
Abstract
Poly-gamma-D-glutamic acid from Bacillus licheniformis is a water-soluble, nontoxic, nonimmunogenic exopolymer. Using synthetic linkers, the alpha-carboxylate side chains of PGA were conjugated to an exposed thiol side chain of an antibody F(ab') fragment, Mc109F4. Analysis of the PGA-Mc109F4 conjugate by gel filtration HPLC revealed a mixture of multivalent conjugates. The PGA-Mc109F4 conjugate retained biological activity, but showed a lower binding affinity to target BCL3B3 cells than free Mc109F4 F(ab')2 by flow cytometry, and a lower efficacy for BCL3B3 growth inhibition than free Mc109F4 F(ab')(2). PGA was also conjugated with the free amino group of glycopeptide antibiotic vancomycin. The PGA-vancomycin conjugate showed slightly lower antibacterial activity than free vancomycin versus susceptible Bacillus subtilis, but slightly higher activity versus intrinsically resistant Leuconostoc mesenteroides.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | BIOCONJUGATE CHEMISTRY | ||||
Publisher: | AMER CHEMICAL SOC | ||||
ISSN: | 1043-1802 | ||||
Official Date: | November 2003 | ||||
Dates: |
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Volume: | 14 | ||||
Number: | 6 | ||||
Number of Pages: | 8 | ||||
Page Range: | pp. 1148-1155 | ||||
DOI: | 10.1021/bc020019m | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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