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Combined two-photon excitation and d→f energy transfer in a water-soluble IrIII/EuIIIDyad : two luminescence components from one molecule for cellular imaging
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Baggaley, Elizabeth, Cao, Deng-Ke, Sykes, Daniel, Botchway, Stanley W., Weinstein, Julia A. and Ward, Michael D. (2014) Combined two-photon excitation and d→f energy transfer in a water-soluble IrIII/EuIIIDyad : two luminescence components from one molecule for cellular imaging. Chemistry - A European Journal, 20 (29). pp. 8898-8903. doi:10.1002/chem.201403618 ISSN 0947-6539.
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Official URL: http://dx.doi.org/10.1002/chem.201403618
Abstract
The first example of cell imaging using two independent emission components from a dinuclear d/f complex is reported. A water-stable, cell-permeable IrIII/EuIII dyad undergoes partial Ir→Eu energy transfer following two-photon excitation of the Ir unit at 780 nm. Excitation in the near-IR region generated simultaneously green Ir-based emission and red Eu-based emission from the same probe. The orders-of-magnitude difference in their timescales (Ir ca. μs; Eu ca. 0.5 ms) allowed them to be identified by time-gated detection. Phosphorescence lifetime imaging microscopy (PLIM) allowed the lifetime of the Ir-based emission to be measured in different parts of the cell. At the same time, the cells are simultaneously imaged by using the Eu-based emission component at longer timescales. This new approach to cellular imaging by using dual d/f emitters should therefore enable autofluorescence-free sensing of two different analytes, independently, simultaneously and in the same regions of a cell.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Journal or Publication Title: | Chemistry - A European Journal | ||||
Publisher: | Wiley - V C H Verlag | ||||
ISSN: | 0947-6539 | ||||
Official Date: | 14 July 2014 | ||||
Dates: |
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Volume: | 20 | ||||
Number: | 29 | ||||
Page Range: | pp. 8898-8903 | ||||
DOI: | 10.1002/chem.201403618 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published |
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