The Library
A kinetic model describing the processivity of Myosin-V
Tools
Skau, Karl I., Hoyle, Rebecca B. and Turner, Matthew S. (2006) A kinetic model describing the processivity of Myosin-V. Biophysical Journal, Vol.91 (No.7). pp. 2475-2489. doi:10.1529/biophysj.105.070888 ISSN 0006-3495.
|
PDF
WRAP_Skau_kinetic_model.pdf - Requires a PDF viewer. Download (273Kb) |
Official URL: http://dx.doi.org/10.1529/biophysj.105.070888
Abstract
The precise details of how myosin-V coordinates the biochemical reactions and mechanical motions of its two head elements to engineer effective processive molecular motion along actin filaments remain unresolved. We compare a quantitative kinetic model of the myosin-V walk, consisting of five basic states augmented by two further states to allow for futile hydrolysis and detachments, with experimental results for run lengths, velocities, and dwell times and their dependence on bulk nucleotide concentrations and external loads in both directions. The model reveals how myosin-V can use the internal strain in the molecule to synchronize the motion of the head elements. Estimates for the rate constants in the reaction cycle and the internal strain energy are obtained by a computational comparison scheme involving an extensive exploration of the large parameter space. This scheme exploits the fact that we have obtained analytic results for our reaction network, e.g., for the velocity but also the run length, diffusion constant, and fraction of backward steps. The agreement with experiment is often reasonable but some open problems are highlighted, in particular the inability of such a general model to reproduce the reported dependence of run length on ADP concentration. The novel way that our approach explores parameter space means that any confirmed discrepancies should give new insights into the reaction network model.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Physics | ||||
Library of Congress Subject Headings (LCSH): | Myosin, Microfilament proteins, Actin, Adenosine diphosphate | ||||
Journal or Publication Title: | Biophysical Journal | ||||
Publisher: | Biophysical Society | ||||
ISSN: | 0006-3495 | ||||
Official Date: | October 2006 | ||||
Dates: |
|
||||
Volume: | Vol.91 | ||||
Number: | No.7 | ||||
Page Range: | pp. 2475-2489 | ||||
DOI: | 10.1529/biophysj.105.070888 | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC) | ||||
Grant number: | GR/S24671/01 (EPSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |