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Protein-ligand and protein-protein interactions studied by electrospray ionization and mass spectrometry
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UNSPECIFIED (2003) Protein-ligand and protein-protein interactions studied by electrospray ionization and mass spectrometry. In: Meeting on Intermolecular Associations in 2D and 3D, UNIV NOTTINGHAM, NOTTINGHAM, ENGLAND, JUN 19-20, 2003. Published in: BIOCHEMICAL SOCIETY TRANSACTIONS, 31 (Part 5). pp. 985-989. ISSN 0300-5127.
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Abstract
Electrospray ionization has made possible the transference of non-covalently bound complexes from solution phase to high vacuum. in the process, a complex acquires a net charge and becomes amenable to measurement by MS. FTICR (Fourier-transform ion cyclotron resonance) MS allows these ions to be measured with sufficiently high resolution for the isotopomers of complexes of small proteins to be resolved from each other (true for complexes up to about 100 kDa for the most powerful FTICR instruments), which is of crucial significance in the interpretation of spectra. Results are presented for members of the S100 family of proteins, demonstrating how non-covalently bound complexes can be distinguished unambiguously from covalently bound species. Consideration relevant both to determination of binding constants in solution from the gas-phase results and to the elucidation of protein folding and unfolding in solution are discussed. The caveats inherent to the basic approach of using electrospray and MS to characterize protein complexes are weighed and evaluated.
Item Type: | Conference Item (UNSPECIFIED) | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | BIOCHEMICAL SOCIETY TRANSACTIONS | ||||
Publisher: | PORTLAND PRESS | ||||
ISSN: | 0300-5127 | ||||
Official Date: | October 2003 | ||||
Dates: |
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Volume: | 31 | ||||
Number: | Part 5 | ||||
Number of Pages: | 5 | ||||
Page Range: | pp. 985-989 | ||||
Publication Status: | Published | ||||
Title of Event: | Meeting on Intermolecular Associations in 2D and 3D | ||||
Location of Event: | UNIV NOTTINGHAM, NOTTINGHAM, ENGLAND | ||||
Date(s) of Event: | JUN 19-20, 2003 |
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