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Diminished expression of an antiviral ribonuclease in response to pneumovirus infection in vivo
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UNSPECIFIED (2003) Diminished expression of an antiviral ribonuclease in response to pneumovirus infection in vivo. ANTIVIRAL RESEARCH, 59 (3). pp. 181-191. doi:10.1016/S0166-3542(03)00111-6 ISSN 0166-3542.
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Official URL: http://dx.doi.org/10.1016/S0166-3542(03)00111-6
Abstract
The mouse eosinophil-associated ribonucleases (mEars) are species specific, divergent orthologs of the human antiviral RNase A ribonucleases, eosinophil-derived neurotoxin (RNase 2) and eosinophil cationic protein (RNase 3). We show here that mEar 2 is also an antiviral ribonuclease, as micromolar concentrations promote a similar tosixfold reduction in the infectivity of pneumonia virus of mice (PVM) for target respiratory epithelial cells in vitro. Although initially identified as a component of eosinophilic leukocytes, mEar 2 mRNA and protein were also detected in lung tissue accompanied by enzymatically active mEar 2 in bronchoalveolar lavage fluid (BALF). At t = 3 days post-inoculation with PVM (strain J3666), we observed the characteristic inflammatory response accompanied by diminished expression of total mEar mRNA and protein in lung tissue and a corresponding fivefold drop in ribonuclease activity in BALF No change in mEar expression was observed in response to infection with PVM strain 15, a replication-competent strain of PVM that does not elicit a cellular inflammatory response. However, mEar expression is not directly dependent on inflammation per se, as diminished expression of mEar mRNA and BAL ribonuclease activity were also observed in PVM-infected, inflammation-deficient, MIP-1alpha -/- mice. We propose that this mechanism may represent a novel virus-mediated evasion strategy, with a mechanism that is linked in some fashion to virus-specific pathogenicity. Published by Elsevier B.V.
Item Type: | Journal Article | ||||
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Subjects: | R Medicine > RS Pharmacy and materia medica Q Science > QR Microbiology > QR355 Virology |
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Journal or Publication Title: | ANTIVIRAL RESEARCH | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0166-3542 | ||||
Official Date: | August 2003 | ||||
Dates: |
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Volume: | 59 | ||||
Number: | 3 | ||||
Number of Pages: | 11 | ||||
Page Range: | pp. 181-191 | ||||
DOI: | 10.1016/S0166-3542(03)00111-6 | ||||
Publication Status: | Published |
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