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Consensus structural features of purified bacterial TatABC complexes
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UNSPECIFIED (2003) Consensus structural features of purified bacterial TatABC complexes. Journal of Molecular Biology, 330 (2). pp. 277-286. doi:10.1016/S0022-2836(03)00621-1 ISSN 0022-2836.
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Official URL: http://dx.doi.org/10.1016/S0022-2836(03)00621-1
Abstract
The twin-arginine translocation (Tat) system transports folded proteins across bacterial plasma membranes and the chloroplast thylakoid membrane. Here, we investigate the composition and structural organization of three different purified Tat complexes from Escherichia coli, Salmonella typhimurium and Agrobacterium tumefaciens. First, we demonstrate the functional activity of these Tat systems in vivo, since expression of the tatABC operons from S. typhimurium or A. tumefaciens in an E. coli tat null mutant strain resulted in efficient Tat-dependent export of an E. coli cofactor-containing substrate, TMAO reductase. The three isolated, affinity-tagged Tat complexes comprised TatA, TatB and TatC in each case, demonstrating a strong interaction between these three subunits. Single-particle electron microscopy studies of all three complexes revealed approximately oval-shaped, asymmetric particles with maximal dimensions up to 13 nm. A common feature is a number of stain-excluding densities surrounding more or less central pools of stain, suggesting protein-lined pores or cavities. The characteristics of size variation among the particles suggest a modular form of assembly and/or the recruitment of varying numbers of TatBC/TatA units. Despite low levels of sequence homology, the combined data indicate structural and functional conservation in the Tat systems of these three bacterial species. (C) 2003 Elsevier Science Ltd. All rights reserved.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | Journal of Molecular Biology | ||||
Publisher: | ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD | ||||
ISSN: | 0022-2836 | ||||
Official Date: | 4 July 2003 | ||||
Dates: |
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Volume: | 330 | ||||
Number: | 2 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 277-286 | ||||
DOI: | 10.1016/S0022-2836(03)00621-1 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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