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Isolation of high molecular weight DNA from soil for cloning into BAC vectors
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UNSPECIFIED (2003) Isolation of high molecular weight DNA from soil for cloning into BAC vectors. FEMS MICROBIOLOGY LETTERS, 223 (1). pp. 15-20. doi:10.1016/S0378-1097(03)00248-9 ISSN 0378-1097.
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Official URL: http://dx.doi.org/10.1016/S0378-1097(03)00248-9
Abstract
Isolation of high molecular weight DNA fragments from soil, in excess of 1 Mb, and of sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination of indirect extraction of cells, using a nycodenz extraction technique, followed by lysis of biomass immobilised in agarose plugs, allowed fragments in excess of 1 Mb to be purified. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | FEMS MICROBIOLOGY LETTERS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0378-1097 | ||||
Official Date: | 6 June 2003 | ||||
Dates: |
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Volume: | 223 | ||||
Number: | 1 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 15-20 | ||||
DOI: | 10.1016/S0378-1097(03)00248-9 | ||||
Publication Status: | Published |
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