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Carbohydrate-protein interactions at interfaces: synthesis of thiolactosyl glycolipids and design of a working model for surface plasmon resonance
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UNSPECIFIED (2003) Carbohydrate-protein interactions at interfaces: synthesis of thiolactosyl glycolipids and design of a working model for surface plasmon resonance. ORGANIC & BIOMOLECULAR CHEMISTRY, 1 (6). pp. 928-938. doi:10.1039/b210672h ISSN 1477-0520.
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Official URL: http://dx.doi.org/10.1039/b210672h
Abstract
Thiolactosyl lipids designed for carbohydrate-protein binding studies have been synthesised. One representative was selected for binding studies with a plant lectin RCA(120), the agglutinin from Ricinus communis. The interactions were measured quantitatively in real time using a BIAcore surface plasmon resonance instrument. Removal of much of the galactose from the thiolactosyl lipid in situ with beta-galactosidase showed that the lectin binding was highly specific. A dissociation constant K-D = 8.77 x 10(-8) M was measured for 1-{2-[2-(2-[beta-D-galactopyranosyl-(1 --> 4)-1-thio-beta-D-glucopyranosyl] ethoxy) ethoxy] ethoxy} octadecane 30 which is four orders of magnitude greater than that determined for binding to lactose in solution. A concentration of lactose of >80 mM was required to block the lectin binding to thiolactosyl lipid in a neomembrane.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | ORGANIC & BIOMOLECULAR CHEMISTRY | ||||
Publisher: | ROYAL SOC CHEMISTRY | ||||
ISSN: | 1477-0520 | ||||
Official Date: | 2003 | ||||
Dates: |
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Volume: | 1 | ||||
Number: | 6 | ||||
Number of Pages: | 11 | ||||
Page Range: | pp. 928-938 | ||||
DOI: | 10.1039/b210672h | ||||
Publication Status: | Published |
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