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UV laser ablation to measure cell and tissue-generated forces in the Zebrafish embryo in vivo and ex vivo
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Smutny, Michael, Behrndt, Martin, Campinho, Pedro, Ruprecht, Verena and Heisenberg, Carl-Philipp (2015) UV laser ablation to measure cell and tissue-generated forces in the Zebrafish embryo in vivo and ex vivo. In: Nelson, Celeste M., (ed.) Tissue Morphogenesis. Methods in Molecular Biology (Methods and Protocols), 1189 . New York, US: Humana Press, pp. 219-235. ISBN 9781493911639
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Official URL: http://dx.doi.org/10.1007/978-1-4939-1164-6_15
Abstract
Mechanically coupled cells can generate forces driving cell and tissue morphogenesis during development. Visualization and measuring of these forces is of major importance to better understand the complexity of the biomechanic processes that shape cells and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively assess mechanical tension in different tissues of the developing zebrafish and in cultures of primary germ layer progenitor cells ex vivo.
Item Type: | Book Item | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Series Name: | Methods in Molecular Biology (Methods and Protocols) | ||||||
Publisher: | Humana Press | ||||||
Place of Publication: | New York, US | ||||||
ISBN: | 9781493911639 | ||||||
ISSN: | 1064-3745 | ||||||
Book Title: | Tissue Morphogenesis | ||||||
Editor: | Nelson, Celeste M. | ||||||
Official Date: | 2015 | ||||||
Dates: |
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Volume: | 1189 | ||||||
Page Range: | pp. 219-235 | ||||||
DOI: | 10.1007/978-1-4939-1164-6_15 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access |
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