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Transactivation of E2F-regulated genes by polyomavirus large T antigen : evidence for a two-step mechanism
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Nemethova, M., Smutny, M. and Wintersberger, E. (2004) Transactivation of E2F-regulated genes by polyomavirus large T antigen : evidence for a two-step mechanism. Molecular and Cellular Biology, 24 (24). pp. 10986-10994. doi:10.1128/MCB.24.24.10986-10994.2004 ISSN 0270-7306.
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Official URL: http://dx.doi.org/10.1128/MCB.24.24.10986-10994.20...
Abstract
Polyomavirus large T antigen transactivates a variety of genes whose products are involved in S phase induction. These genes are regulated by the E2F family of transcription factors, which are under the control of the pocket protein retinoblastoma protein and its relatives p130 and p107. The viral protein causes a dissociation of E2F-pocket protein complexes that results in transactivation of the genes. This reaction requires the N-terminal binding site for pocket proteins and the J domain that binds chaperones. We found earlier that a mutation of the zinc finger located within the C-terminal domain, a region assumed to function mainly in the replication of viral DNA, also interferes with transactivation. Here we show that binding of the histone acetyltransferase coactivator complex CBP/p300-PCAF to the C terminus correlates with the ability of large T antigen to transactivate genes. This interaction results in promoter-specific acetylation of histones. Inactive mutant proteins with changes within the C-terminal domain were nevertheless able to dissociate the E2F pocket protein complexes, indicating that this dissociation is a necessary but insufficient step in the T antigen-induced transactivation of genes. It has to be accompanied by a second step involving the T antigen-mediated recruitment of a histone acetyltransferase complex.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Molecular and Cellular Biology | ||||
Publisher: | American Society for Microbiology | ||||
ISSN: | 0270-7306 | ||||
Official Date: | 2004 | ||||
Dates: |
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Volume: | 24 | ||||
Number: | 24 | ||||
Page Range: | pp. 10986-10994 | ||||
DOI: | 10.1128/MCB.24.24.10986-10994.2004 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) |
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