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Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction
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Chew, Ting Gang, Huang, Junqi, Palani, Saravanan, Sommese, Ruth, Kamnev, Anton, Hatano, Tomoyuki, Gu, Ying, Oliferenko, Snezhana, Sivaramakrishnan, Sivaraj and Balasubramanian, Mohan K. (2017) Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction. The Journal of Cell Biology, 216 (9). pp. 2657-2667. doi:10.1083/jcb.201701104 ISSN 1540-8140.
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Official URL: http://dx.doi.org/10.1083/jcb.201701104
Abstract
Cytokinesis in many eukaryotes involves a tension-generating actomyosin-based contractile ring. Many components of actomyosin rings turn over during contraction, although the significance of this turnover has remained enigmatic. Here, using Schizosaccharomyces japonicus, we investigate the role of turnover of actin and myosin II in its contraction. Actomyosin ring components self-organize into ∼1-µm-spaced clusters instead of undergoing full-ring contraction in the absence of continuous actin polymerization. This effect is reversed when actin filaments are stabilized. We tested the idea that the function of turnover is to ensure actin filament homeostasis in a synthetic system, in which we abolished turnover by fixing rings in cell ghosts with formaldehyde. We found that these rings contracted fully upon exogenous addition of a vertebrate myosin. We conclude that actin turnover is required to maintain actin filament homeostasis during ring contraction and that the requirement for turnover can be bypassed if homeostasis is achieved artificially.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Cytokinesis -- Molecular aspects, Actin, Homeostasis, Actomyosin | |||||||||||||||
Journal or Publication Title: | The Journal of Cell Biology | |||||||||||||||
Publisher: | Rockefeller University Press | |||||||||||||||
ISSN: | 1540-8140 | |||||||||||||||
Official Date: | 4 September 2017 | |||||||||||||||
Dates: |
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Volume: | 216 | |||||||||||||||
Number: | 9 | |||||||||||||||
Page Range: | pp. 2657-2667 | |||||||||||||||
DOI: | 10.1083/jcb.201701104 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 12 June 2018 | |||||||||||||||
Date of first compliant Open Access: | 12 June 2018 | |||||||||||||||
RIOXX Funder/Project Grant: |
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