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Enhancing the resolution of micro free flow electrophoresis through spatially controlled sample injection
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Saar, Kadi Liis, Müller, Thomas, Charmet, Jérôme, Challa, Pavan K. and Knowles, Tuomas P. J. (2018) Enhancing the resolution of micro free flow electrophoresis through spatially controlled sample injection. Analytical Chemistry, 90 (15). pp. 8998-9005. doi:10.1021/acs.analchem.8b01205 ISSN 1520-6882.
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Official URL: http://dx.doi.org/10.1021/acs.analchem.8b01205
Abstract
Free flow electrophoresis is a versatile technique for the continuous separation of mixtures with both preparative and analytical applications. Microscale versions of free flow electrophoresis are particularly attractive strategies because of their fast separation times, ability to work with small sample volumes and large surface area to volume ratios facilitating rapid heat transfer, thus minimising the detrimental effects of Joule heating even at high voltages. The resolution of microscale free flow electrophoresis, however, is limited by the broadening of the analyte beam in the microfluidic channel - an effect that becomes especially pronounced when the analyte is deflected significantly away from its original position. Here we describe and demonstrate how by spatially restricting the sample injection and collection to the regions where the gradients in the velocity distribution of the carrier medium are the smallest, this broadening effect can be substantially suppressed and hence the resolution of microscale free flow electrophoresis devices increased. To demonstrate this concept we fabricated microfluidic free flow electrophoresis devices with spatially restricted injection nozzles implemented via the use of multilayer soft-photolithography and further integrated quartz based observation areas for fluorescent detection and imaging. With these devices we demonstrated a five fold reduction in the beam broadening extent compared to conventional free flow electrophoresis approaches with non-restricted sample introduction. The manifold enhancement in the achievable resolution of microscale free flow electrophoresis devices opens up the possibility of rapid separation and analysis of more complex mixtures.
Item Type: | Journal Article | ||||||||||||
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Subjects: | Q Science > QD Chemistry T Technology > TJ Mechanical engineering and machinery |
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > WMG (Formerly the Warwick Manufacturing Group) | ||||||||||||
Library of Congress Subject Headings (LCSH): | Electrophoresis, Microfluidics | ||||||||||||
Journal or Publication Title: | Analytical Chemistry | ||||||||||||
Publisher: | American Chemical Society | ||||||||||||
ISSN: | 1520-6882 | ||||||||||||
Official Date: | 7 August 2018 | ||||||||||||
Dates: |
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Volume: | 90 | ||||||||||||
Number: | 15 | ||||||||||||
Page Range: | pp. 8998-9005 | ||||||||||||
DOI: | 10.1021/acs.analchem.8b01205 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
Date of first compliant deposit: | 26 June 2018 | ||||||||||||
Date of first compliant Open Access: | 25 June 2019 | ||||||||||||
RIOXX Funder/Project Grant: |
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