Webber, P. M. (1993) Analysis of the promoter region of the Xenopus borealis N-Cadherin gene. PhD thesis, University of Warwick.

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Abstract

A Xenopus borealis genomic library was screened with the 5'-end of the Xenopus laevis N-Cadherin cDNA (DETRICK et a/., 1990). Four groups of clones were isolated that differed in restriction-enzyme digestion patterns.

The sequencing of one of these clones, 3-9/4.8BS/pBS, has identified regions of DNA highly homologous to the X.laevis N-Cadherin gene. Accordingly, it is believed that the clone 3-9/4.8BS/pBS contains the 5'-end and promoter region of the X.boreal is N-Cadherin gene.

A sequence analysis of this region has shown it to be GC-rich and has revealed consensus TATA-box, CCAAT-box and Spl binding sites. Other possible transcription-factor binding-sites have also been identified, as well as the first exon/intron boundary.

A series of promoter-deletions were fused to the bacterial 3- galactosidase gene and micro-injected into X.laevis embryos. The 3- galactosidase staining patterns of whole embryos has visually shown that 1.3Kb of genomic DNA upstream of the translational start-site is sufficient to direct neural-specific transcription of this reporter gene.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QH Natural history > QH426 Genetics
Library of Congress Subject Headings (LCSH):	Xenopus -- Genetics, Cadherins, Promoters (Genetics), Sequence alignment (Bioinformatics)
Official Date:	August 1993
Dates:	Date Event August 1993 Submitted
Institution:	University of Warwick
Theses Department:	Department of Biological Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Sponsors:	Medical Research Council (Great Britain)
Format of File:	pdf
Extent:	ix, 326 leaves : illustrations, charts
Language:	eng

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