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The influence of the growth environment on the stability of a drug resistant plasmid in Escherichia coli K12 grown in chemostat culture
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Godwin, Dianne (1980) The influence of the growth environment on the stability of a drug resistant plasmid in Escherichia coli K12 grown in chemostat culture. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b1751767~S15
Abstract
Populations of Escherichia coli K12 containing the plasmid TP120 which coded for resistance to ampicillin, streotomycin, sulphonamide and tetracycline were grown in continuous culture under carbon-limited and phosphor us-limited conditions. The maintenance of the plasmid TP120 was studied and it was discovered that with time, resistance to one or more of the drugs was lost, resulting in the production of mutant populations which were more competitive than the parent population. The tetracycline resistance marker was always lost under carbon and phosphor us-limitations, but resistance to the other three drugs was lost only under phosphate- limited growth. A plasmid-minus strain was isolated on only one occasion. Strains of E. coli which had lost resistance to one or more drugs were shown to be smaller in size than the parent strain and were capable of higher maximum specific growth rates than the parent strain. Mixed culture competition experiments also demonstrated that evolved strains had lower Kq values than the original, parent strain. However the rate of carbon and phosphor U3 uptake measured in radiochemical experiments was unchanged for parent and evolved strains. The significance of plasmid stability within populations of bacteria in relation to the natural situation was also discussed.
| Item Type: | Thesis (PhD) | ||||
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| Subjects: | Q Science > QR Microbiology | ||||
| Library of Congress Subject Headings (LCSH): | Drug resistance in microorganisms, Escherichia coli, Plasmids, Bacteria | ||||
| Official Date: | 1980 | ||||
| Dates: |
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| Institution: | University of Warwick | ||||
| Theses Department: | School of Life Sciences | ||||
| Thesis Type: | PhD | ||||
| Publication Status: | Unpublished | ||||
| Supervisor(s)/Advisor: | Slater, J. H. | ||||
| Sponsors: | Medical Research Council (Great Britain) | ||||
| Extent: | 372, [11] leaves : charts | ||||
| Language: | eng |
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