Smith, Steve, 1952- (1980) Synthesis of the small subunit of ribulose-1,5-bisphosphate carboxylase. PhD thesis, University of Warwick.

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Abstract

The synthesis of the small subunit of ribulose-1,5-bisphosphate carboxylase from leaves of pea (Pisum sativum) and its assembly into the holoenzyme have been reconstituted in cell-free extracts. The small subunit precursor has been synthesised by translating its messenger RNA in a cell-free protein-synthesising extract derived from wheat-germ. This precursor has been processed by a soluble extract from isolated chloroplasts, and the processed molecule shown to assemble into the holoenzyme.

Features of the synthesis and processing of this polypeptide are discussed both in relation to the synthesis of other proteins which are transported across membranes, and in relation to chloroplast development.

Synthetic DNA molecules encoding the messenger RNA for the small subunit precursor have been.Isolated by molecular cloning in a plasmid of Escher ich ia coli. These cloned molecules have been employed to characterise, and to purify, the messenger RNA of the small subunit precursor. The cloned DNA molecules have also been employed to probe pea leaf nuclear RNA for complementary sequences. These studies suggest that the messenger RNA may be first synthesised as a precursor of nearly twice the final size. In etiolated pea leaves, where rlbulose-1,5-bIsphosphate carboxylase is present In reduced amounts relative to green tissue, the messenger RNA for the small subunit precursor has also been shown to be present in reduced amounts. Furthermore nuclei isolated from etiolated tissue contain reduced amounts of transcripts of the small subunit gene. These results suggest that the expression of the gene for the small subunit of rlbulose-1,5-bisphosphate carboxylase may be controlled at the level of transcription.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QK Botany
Library of Congress Subject Headings (LCSH):	Chloroplast DNA
Official Date:	1980
Dates:	Date Event 1980 UNSPECIFIED
Institution:	University of Warwick
Theses Department:	Department of Biological Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Ellis, John
Format of File:	pdf
Extent:	xvii, 297 leaves : illustrations
Language:	eng

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