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Attachment of bacteria to glass surfaces in pure culture and in mixed suspensions and the effect of growth conditions on that attachment
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Neilson, James (1991) Attachment of bacteria to glass surfaces in pure culture and in mixed suspensions and the effect of growth conditions on that attachment. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b3226905~S15
Abstract
The attachment of selected freshwater bacteria, Aeromonas Chromobacterium, a coryneform and Staphylococcus In pure culture and in mixed suspensions with one other bacterium to glass surfaces was investigated in the laboratory.
Changes in the nutrient conditions of the growth medium during growth and attachment and the temperature, pH and electrolyte type and concentration present in the attachment solution during attachment experiments all influenced bacterial attachment. The pure culture attachment results obtained depended on the bacterial species being investigated. When bacterial species were attached in the presence of one other species the growth conditions still had a profound effect on attachment. The results obtained depended on the bacterial species present in the mixed suspension, with some bacterial species having a more profound effect on the attachment of other species than others.
The bacterial species used to study bacterial attachment in the laboratory were used along with Acinetobacter and Pseudomonas to investigate bacterial attachment in vivo using a model system. The attachment of these bacteria were investigated in two- to six-membered suspensions. The biofilms obtained in the model system consisted of smaller bacterial cells in a more densely packed biofilm. These biofilms could still be influenced by growth conditions as temperature was seen to influence the bacterial biofilm obtained. During these attachment experiments the Acinetobacter which did not attach itself had a profound effect on the attachment of other bacterial species when present in the liquid phase.
The mechanism by which Acinetobacter inhibited the attachment of other bacteria was investigated and it appeared Acinetobacter did not excreate a chemical to influence the attachment of other species, but the Acinetobacter cells themselves had to be present in the liquid phase to influence the attachment of other species.
The effects of different molecular weight fractions of Tocil Lake water were investigated for their effect on bacterial attachment. The fraction containing the > 30,000 MV component was found to influence bacterial attachment. The results obtained, depended on the bacterial species being investigated.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Library of Congress Subject Headings (LCSH): | Bacteria -- Adhesion, Bacterial growth, Biofilms, Acinetobacter, Water -- Microbiology | ||||
Official Date: | August 1991 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | Department of Biological Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Fletcher, Madilyn ; Flint, Ken P. | ||||
Sponsors: | Natural Environment Research Council (Great Britain) | ||||
Format of File: | |||||
Extent: | xx, 253 leaves : charts | ||||
Language: | eng |
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