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Type II phosphatidylinositol 4-kinase β is an integral signaling component of early T cell activation mechanisms
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Sinha, Ranjeet K., Bojjireddy, Naveen, Kulkarni, Dakshayini, Ratheesh, Aparna, Chiplunkar, S.V., Gude, Rajiv and Subrahmanyam, Gosukonda (2013) Type II phosphatidylinositol 4-kinase β is an integral signaling component of early T cell activation mechanisms. Biochimie, 95 (8). pp. 1560-1566. doi:10.1016/j.biochi.2013.04.005 ISSN 0300-9084.
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Official URL: http://dx.doi.org/10.1016/j.biochi.2013.04.005
Abstract
The early signaling events in T cell activation through CD3 receptor include a rapid change in intra cellular free calcium concentration and reorganization of actin cytoskeleton. Phosphatidylinositol 4-kinases (PtdIns 4-kinases) are implicated as key components in these early signaling events. The role of type II PtdIns 4-kinase β in CD3 receptor signaling was investigated with the help of short hairpin RNA sequences. Cross-linking of CD3 receptors on Jurkat T Cells with monoclonal antibodies showed an early increase in type II PtdIns 4-kinase activity and co-localization of type II PtdIns 4-kinase β with CD3 ζ. Transfection of Jurkat T Cells with shRNAs inhibited CD3 receptor mediated type II PtdIns 4-kinase activation with a concomitant reduction in intra cellular calcium release, suggesting a role for type II PtdIns 4-kinase β in CD3 receptor signal transduction. Knock-down of type II PtdIns 4-kinase β with shRNAs also correlated with a decrease in PtdIns 4-kinase activity in cytoskeleton fractions and reduced adhesion to matrigel surfaces. These results indicate that type II PtdIns 4-kinase β is a key component in early T cell activation signaling cascades.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Biochimie | ||||||||
Publisher: | Elsevier Masson | ||||||||
ISSN: | 0300-9084 | ||||||||
Official Date: | August 2013 | ||||||||
Dates: |
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Volume: | 95 | ||||||||
Number: | 8 | ||||||||
Page Range: | pp. 1560-1566 | ||||||||
DOI: | 10.1016/j.biochi.2013.04.005 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access |
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