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An investigation into cell surface variation during the early development of "Xenopus laevis"
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Lewis, Jane Rachel, 1963 (1989) An investigation into cell surface variation during the early development of "Xenopus laevis". PhD thesis, University of Warwick.
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WRAP_Theses_Lewis_1989.pdf - Submitted Version - Requires a PDF viewer. Download (8Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b3229695~S15
Abstract
Membrane proteins in Xenopus laevis embryos were analysed on 1- and 2-dimensional gels. Proteins showing temporal variation in their expression in the membrane during Xenopus development were detected. The overall range of membrane proteins present between fertilisation and late neurula stage embryos was found to stay essentially constant, with few proteins showing temporal variation.
Microinjection of 30 S-methionine into Xenopus laevis embryos was used to investigate membrane protein synthesis. Dramatic changes in the composition of proteins synthesised during Xenopus laevis early development were noted, with most significant changes observed at the gastrula stage following the mid-blastula transition.
Cell membrane variation in Xenopus laevis embryos was studied using cell surface labelling with 125 Iodine. Two proteins specific to labelling of disaggregated embryos were detected and these are thought to be specific to intercellular membranes. Cell surface labelling with 125 Iodine was also used to characterise overall changes in the cell membrane during Xenopus laevis early development. Histo- autoradiography of 125 Iodine surface labelled embryos was used to confirm the origin of cleavage furrow membrane in the dividing embryo, and the source of ciliated cells from the double layered epidermis in Xenopus laevis neurulae.
2B12, a monoclonal antibody raised against adult Xenopus laevis brain tissue, was characterised on embryo and tissue sections using indirect immunofluoresence. Biochemical analysis of 2B12 activity has indicated that the antigen may be a ganglioside, specific to myelin and other nervous tissue membrane.
| Item Type: | Thesis (PhD) | ||||
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| Subjects: | Q Science > QH Natural history Q Science > QL Zoology |
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| Library of Congress Subject Headings (LCSH): | Xenopus laevis, Cell membranes, Cell membranes -- Variation | ||||
| Official Date: | January 1989 | ||||
| Dates: |
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| Institution: | University of Warwick | ||||
| Theses Department: | Department of Biological Sciences | ||||
| Thesis Type: | PhD | ||||
| Publication Status: | Unpublished | ||||
| Supervisor(s)/Advisor: | Jones, E. A. | ||||
| Format of File: | |||||
| Extent: | xxv, 326 leaves : illustrations | ||||
| Language: | eng |
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