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Albugo candida race diversity, ploidy and host-associated microbes revealed using DNA sequence capture on diseased plants in the field
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Jouet, Agathe, Saunders, Diane G. O., McMullan, Mark, Ward, Ben, Furzer, Oliver, Jupe, Florian, Cevik, Volkan, Hein, Ingo, Thilliez, Gaetan J. A., Holub, E. B., van Oosterhout, Cock and Jones, Jonathan D. G. (2019) Albugo candida race diversity, ploidy and host-associated microbes revealed using DNA sequence capture on diseased plants in the field. New Phytologist, 221 (3). pp. 1529-1543. doi:10.1111/nph.15417 ISSN 0028-646X.
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Official URL: http://dx.doi.org/10.1111/nph.15417
Abstract
• Physiological races of the oomycete Albugo candida are biotrophic pathogens of diverse plant species, primarily the Brassicaceae, and cause infections that suppress host immunity to other pathogens. However, A. candida race diversity and the consequences of host immunosuppression are poorly understood in the field.
• We report a method that enables sequencing of DNA of plant pathogens and plantassociated microbes directly from field samples (Pathogen Enrichment Sequencing: PenSeq). We apply this method to explore race diversity in A. candida and to detect A. candidaassociated microbes in the field (91 A. candida-infected plants).
• We show with unprecedented resolution that each host plant species supports colonization by one of 17 distinct phylogenetic lineages, each with an unique repertoire of effector candidate alleles. These data reveal the crucial role of sexual and asexual reproduction, polyploidy and host domestication in A. candida specialization on distinct plant species. Our bait design also enabled phylogenetic assignment of DNA sequences from bacteria and fungi from plants in the field.
• This paper shows that targeted sequencing has a great potential for the study of pathogen populations while they are colonizing their hosts. This method could be applied to other microbes, especially to those that cannot be cultured.
| Item Type: | Journal Article | |||||||||||||||||||||||||||
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| Subjects: | Q Science > QK Botany | |||||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Albugo candida -- Genetics, Nucleotide sequence, Plant defenses, Immunosuppression, Plant diseases -- Genetic aspects, Oomycetes -- Genetics | |||||||||||||||||||||||||||
| Journal or Publication Title: | New Phytologist | |||||||||||||||||||||||||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | |||||||||||||||||||||||||||
| ISSN: | 0028-646X | |||||||||||||||||||||||||||
| Official Date: | February 2019 | |||||||||||||||||||||||||||
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| Volume: | 221 | |||||||||||||||||||||||||||
| Number: | 3 | |||||||||||||||||||||||||||
| Page Range: | pp. 1529-1543 | |||||||||||||||||||||||||||
| DOI: | 10.1111/nph.15417 | |||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||
| Date of first compliant deposit: | 6 November 2018 | |||||||||||||||||||||||||||
| Date of first compliant Open Access: | 7 November 2018 | |||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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