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Cell cycle constraints on capsulation and bacteriophage susceptibility
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Ardissone, Silvia, Fumeaux, Coralie, Bergé, Matthieu, Beaussart, Audrey, Théraulaz, Laurence, Radhakrishnan, Sunish Kumar, Dufrêne, Yves F. and Viollier, Patrick H. (2014) Cell cycle constraints on capsulation and bacteriophage susceptibility. eLife, 3 . e03587. doi:10.7554/eLife.03587 ISSN 2050-084X.
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Official URL: http://dx.doi.org/10.7554/eLife.03587
Abstract
Despite the crucial role of bacterial capsules in pathogenesis, it is still unknown if systemic cues such as the cell cycle can control capsule biogenesis. In this study, we show that the capsule of the synchronizable model bacterium Caulobacter crescentus is cell cycle regulated and we unearth a bacterial transglutaminase homolog, HvyA, as restriction factor that prevents capsulation in G1-phase cells. This capsule protects cells from infection by a generalized transducing Caulobacter phage (φCr30), and the loss of HvyA confers insensitivity towards φCr30. Control of capsulation during the cell cycle could serve as a simple means to prevent steric hindrance of flagellar motility or to ensure that phage-mediated genetic exchange happens before the onset of DNA replication. Moreover, the multi-layered regulatory circuitry directing HvyA expression to G1-phase is conserved during evolution, and HvyA orthologues from related Sinorhizobia can prevent capsulation in Caulobacter, indicating that alpha-proteobacteria have retained HvyA activity.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
Journal or Publication Title: | eLife | ||||
Publisher: | eLife Sciences Publications Ltd. | ||||
ISSN: | 2050-084X | ||||
Official Date: | 25 November 2014 | ||||
Dates: |
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Volume: | 3 | ||||
Article Number: | e03587 | ||||
DOI: | 10.7554/eLife.03587 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) |
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