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Engineered K1F bacteriophages kill intracellular Escherichia coli K1 in human epithelial cells
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Møller-Olsen, Christian, Ho, Siu Fung, Shukla, Ranti Dev, Feher, Tamas and Sagona, Antonia P. (2018) Engineered K1F bacteriophages kill intracellular Escherichia coli K1 in human epithelial cells. Scientific Reports, 8 . 17559. doi:10.1038/s41598-018-35859-6 ISSN 2045-2322.
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Official URL: https://doi.org/10.1038/s41598-018-35859-6
Abstract
Bacterial infections can be treated with bacteriophages that show great specificity towards their bacterial host and can be genetically modified for different applications. However, whether and how bacteriophages can kill intracellular bacteria in human cells remains elusive. Here, using CRISPR/Cas selection, we have engineered a fluorescent bacteriophage specific for E. coli K1, a nosocomial pathogen responsible for urinary tract infections, neonatal meningitis and sepsis. By confocal and live microscopy, we show that engineered bacteriophages K1F-GFP and E. coli EV36-RFP bacteria displaying the K1 capsule, enter human cells via phagocytosis. Importantly, we show that bacteriophage K1F-GFP efficiently kills intracellular E. coli EV36-RFP in T24 human urinary bladder epithelial cells. Finally, we provide evidence that bacteria and bacteriophages are degraded by LC3-associated phagocytosis and xenophagy.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QR Microbiology R Medicine > RC Internal medicine |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Bacterial diseases -- Treatment, Urinary tract infections, Meningitis, Septicemia, Escherichia coli, Epithelial cells | ||||||||||||||||||
Journal or Publication Title: | Scientific Reports | ||||||||||||||||||
Publisher: | Nature Publishing Group | ||||||||||||||||||
ISSN: | 2045-2322 | ||||||||||||||||||
Official Date: | 3 December 2018 | ||||||||||||||||||
Dates: |
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Volume: | 8 | ||||||||||||||||||
Article Number: | 17559 | ||||||||||||||||||
DOI: | 10.1038/s41598-018-35859-6 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 3 December 2018 | ||||||||||||||||||
Date of first compliant Open Access: | 3 December 2018 | ||||||||||||||||||
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