The Library
Coping with environmental eukaryotes : identification of Pseudomonas syringae genes during the interaction with alternative hosts or predators
Tools
Dorati, Federico, Barrett, Glyn, Sanchez-Contreras, Maria, Arseneault, Tanya, José, Mateo, Studholme, David, Murillo, Jesús, Caballero, Primitivo, Waterfield, Nicholas R., Arnold, Dawn, Shaw, Liz and Jackson, Robert (2018) Coping with environmental eukaryotes : identification of Pseudomonas syringae genes during the interaction with alternative hosts or predators. Microorganisms, 6 (2). 32. doi:10.3390/microorganisms6020032 ISSN 2076-2607.
|
PDF
WRAP-coping-environmental-eukaryotes-Waterfield-2018.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3272Kb) | Preview |
Official URL: http://dx.doi.org/10.3390/microorganisms6020032
Abstract
Understanding the molecular mechanisms underpinning the ecological success of plant pathogens is critical to develop strategies for controlling diseases and protecting crops. Recent observations have shown that plant pathogenic bacteria, particularly Pseudomonas, exist in a range of natural environments away from their natural plant host e.g., water courses, soil, non-host plants. This exposes them to a variety of eukaryotic predators such as nematodes, insects and amoebae present in the environment. Nematodes and amoeba in particular are bacterial predators while insect herbivores may act as indirect predators, ingesting bacteria on plant tissue. We therefore postulated that bacteria are probably under selective pressure to avoid or survive predation and have therefore developed appropriate coping mechanisms. We tested the hypothesis that plant pathogenic Pseudomonas syringae are able to cope with predation pressure and found that three pathovars show weak, but significant resistance or toxicity. To identify the gene systems that contribute to resistance or toxicity we applied a heterologous screening technique, called Rapid Virulence Annotation (RVA), for anti-predation and toxicity mechanisms. Three cosmid libraries for P. syringae pv. aesculi, pv. tomato and pv. phaseolicola, of approximately 2000 cosmids each, were screened in the susceptible/non-toxic bacterium Escherichia coli against nematode, amoebae and an insect. A number of potential conserved and unique genes were identified which included genes encoding haemolysins, biofilm formation, motility and adhesion. These data provide the first multi-pathovar comparative insight to how plant pathogens cope with different predation pressures and infection of an insect gut and provide a foundation for further study into the function of selected genes and their role in ecological success.
Item Type: | Journal Article | ||||||
---|---|---|---|---|---|---|---|
Subjects: | Q Science > QK Botany Q Science > QR Microbiology |
||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||||
Library of Congress Subject Headings (LCSH): | Plant diseases -- Genetic aspects, Pseudomonas syringae, Bacterial diseases of plants, Virulence (Microbiology), Caenorhabditis elegans, Acanthamoeba, Greater wax moth, Phytopathogenic microorganisms -- Control | ||||||
Journal or Publication Title: | Microorganisms | ||||||
Publisher: | MDPI | ||||||
ISSN: | 2076-2607 | ||||||
Official Date: | 21 April 2018 | ||||||
Dates: |
|
||||||
Volume: | 6 | ||||||
Number: | 2 | ||||||
Article Number: | 32 | ||||||
DOI: | 10.3390/microorganisms6020032 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 28 November 2018 | ||||||
Date of first compliant Open Access: | 28 November 2018 | ||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year